Expression of a Drosophila melanogaster acetylcholine receptor-related gene in the central nervous system
The authors isolated Drosophila melanogaster genomic sequences with nucleotide and amino acid sequence homology to subunits of vertebrate acetylcholine receptor by hybridization with a Torpedo acetylcholine receptor subunit cDNA probe. Five introns are present in the portion of the Drosophila gene encoding the unprocessed protein and are positionally conserved relative to the human acetylcholine receptor alpha-subunit gene. The Drosophila genomic clone hybridized to salivary gland polytene chromosome 3L within region 64B and was termed AChR64B. A 3-kilobasae poly(A)-containing transcript complementary to the AChR64B clone was readily detectable by RNA blot hybridizations during midembryogenesis, during metamorphosis, and in newly enclosed adults. AChR64B transcripts were localized to the cellular regions of the central nervous system during embryonic, larval, pupal, and adult stages of development. During metamorphosis, a temporal relationship between the morphogenesis of the optic lobe and expression of AChR64B transcripts was observed.
- Research Organization:
- Cell Biology Group, Worcester Foundation for Experimental Biology, 222 Maple Avenue, Shrewsbury, MA 01545 (US)
- OSTI ID:
- 5113796
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genetic characterization and cloning of Mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster
Cytogenetic and molecular localization of tipE: A gene affecting sodium channels in Drosophila melanogaster
Related Subjects
ACETYLCHOLINE
RECEPTORS
DROSOPHILA
GENETIC MAPPING
AMINO ACID SEQUENCE
CENTRAL NERVOUS SYSTEM
GENE RECOMBINATION PROTEINS
GENES
HYBRIDIZATION
ONTOGENESIS
PHENOTYPE
TRANSCRIPTION
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARTHROPODS
AUTONOMIC NERVOUS SYSTEM AGENTS
DIPTERA
DRUGS
ESTERS
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
MAPPING
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
550200* - Biochemistry