A 4-kDa maize chloroplast polypeptide associated with the cytochrome b sub 6 -f complex: Subunit 5, encoded by the chloroplast petE gene

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.5.1534· OSTI ID:5104138

Haley, J; Bogorad, L ^[1]

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1. Harvard Univ., Cambridge, MA (USA)

Four polypeptides, three of which are chloroplast-encoded, have been shown to be associated with the thylakoid membrane cytochrome b{sub 6}-f complex. In this report, the gene for a fifth polypeptide, which copurifies with the b{sub 6}-f complex, is identified through the use of an antibody generated against a synthetic decapeptide predicted from a maize chloroplast DNA sequence. The deduced 37-amino acid sequence of the immunoreactive 4-kDa polypeptide is 100% and 86% conserved in the respective similar open reading frames encoded by Nicotiana tabacum and Marchantia chloroplast DNA. The 4-kDa polypeptide is present in both etioplasts and chloroplasts of maize and is found as well in spinach, tobacco, pea, wheat, and rice thylakoids. Similar to the other subunits of the b{sub 6}-f complex, it is intrinsic to the membrane, and its hydrophilic COOH terminus is located at the stromal thylakoid surface. The authors propose to call the 4-kDa polypeptide subunit 5 and the chloroplast gene that encodes it the petE gene.

• OSTI ID: 5104138
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:5; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
GENES
DNA SEQUENCING
MAIZE
CHLOROPLASTS
POLYPEPTIDES
AMINO ACID SEQUENCE
CYTOCHROMES
ELECTRON TRANSFER
PEAS
PHOTOSYNTHETIC REACTION CENTERS
RECOMBINANT DNA
RICE
SPINACH
TOBACCO
WHEAT
CELL CONSTITUENTS
CEREALS
DNA
FOOD
GRASS
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PIGMENTS
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)