Eel calcitonin binding site distribution and antinociceptive activity in rats

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(86)90230-5· OSTI ID:5096101

Guidobono, F; Netti, C; Sibilia, V; Villa, I; Zamboni, A; Pecile, A

The distribution of binding site for (/sup 125/I)-eel-calcitonin (ECT) to rat central nervous system, studied by an autoradiographic technique, showed concentrations of binding in the diencephalon, the brain stem and the spinal cord. Large accumulations of grains were seen in the hypothalamus, the amygdala, in the fasciculus medialis prosencephali, in the fasciculus longitudinalis medialis, in the ventrolateral part of the periventricular gray matter, in the lemniscus medialis and in the raphe nuclei. The density of grains in the reticular formation and in the nucleus tractus spinalis nervi trigemini was more moderate. In the spinal cord, grains were scattered throughout the dorsal horns. Binding of the ligand was displaced equally by cold ECT and by salmon CT(sCT), indicating that both peptides bind to the same receptors. Human CT was much weaker than sCT in displacing (/sup 125/I)-ECT binding. The administration of ECT into the brain ventricles of rats dose-dependently induced a significant and long-lasting enhancement of hot-plate latencies comparable with that obtained with sCT. The antinociceptive activity induced by ECT is compatible with the topographical distribution of binding sites for the peptide and is a further indication that fish CTs are active in the mammalian brain.

• Research Organization: Univ. of Milan, Italy
• OSTI ID: 5096101
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
CALCITONIN
RADIORECEPTOR ASSAY
PAIN
RATS
RECEPTORS
TISSUE DISTRIBUTION
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
HORMONES
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RODENTS
SYMPTOMS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques