Replication of UV-irradiated single-stranded DNA by DNA polymerase III holoenzyme of Escherichia coli: evidence for bypass of pyrimidine photodimers

Journal Article · Tue Jul 01 00:00:00 EDT 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.13.4599· OSTI ID:5092115

Livneh, Z

Replication of UV-irradiated circular single-stranded phage M13 DNA by Escherichia coli RNA polymerase (EC 2.7.7.6) and DNA polymerase III holoenzyme (EC 2.7.7.7) in the presence of single-stranded DNA binding protein yielded full-length as well as partially replicated products. A similar result was obtained with phage G4 DNA primed with E. coli DNA primase, and phage phi X174 DNA primed with a synthetic oligonucleotide. The fraction of full-length DNA was several orders of magnitude higher than predicted if pyrimidine photodimers were to constitute absolute blocks to DNA replication. Recent models have suggested that pyrimidine photodimers are absolute blocks to DNA replication and that SOS-induced proteins are required to allow their bypass. Our results demonstrate that, under in vitro replication conditions, E. coli DNA polymerase III holoenzyme can insert nucleotides opposite pyrimidine dimers to a significant extent, even in the absence of SOS-induced proteins.

• OSTI ID: 5092115
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 13
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPLICATION
BIOLOGICAL PATHWAYS
BACTERIOPHAGES
DNA POLYMERASES
DNA REPAIR
ESCHERICHIA COLI
PYRIMIDINE DIMERS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
ENZYMES
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATIONS
RECOVERY
REPAIR
TRANSFERASES
VIRUSES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)