Ethanol stimulates phosphoinositide-specific phospholipase C in rat hepatocytes
Ethanol (50-300 mM) causes a transient mobilization of calcium from hormone-sensitive pools in rat hepatocytes. They have studied the action of ethanol on polyphosphoinositide and inositol phosphate levels in these cells. Addition of ethanol to isolated hepatocytes prelabelled with /sup 32/Pi resulted in a small (3-5%) decrease in the level of (/sup 32/P)-phosphatidylinositol 4,5-bisphosphate and a 10-15% increase in (/sup 32/P) phosphatidylinositol 4-phosphate and (/sup 32/P) phosphatidic acid. Maximal changes were seen within 30 sec after ethanol. (/sup 32/P) phosphatidylcholine was unaffected. The changes were concentration dependent over a range of 50-500 mM ethanol. Phorbol esters, which inhibit ethanol-induced calcium mobilization, also inhibited these effects of ethanol on polyphosphoinositides. In intact hepatocytes labeled with myo-(2-/sup 3/H)inositol a significant ethanol-induced increase in (/sup 3/H)inositol phosphates could not be detected. After permeabilization of the cells with digitonin, however, ethanol caused a time and concentration dependent release of (/sup 3/H) inositol trisphosphate and (/sup 3/H)inositol bisphosphate. These results indicate that ethanol-induced calcium mobilization in hepatocytes is due to the activation by ethanol of the phosphoinositide-specific phospholipase C.
- Research Organization:
- Hahnemann Univ. School of Medicine, Philadelphia, PA
- OSTI ID:
- 5090237
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ETHANOL
BIOLOGICAL EFFECTS
LIPASE
ENZYME ACTIVITY
PHORBOL ESTERS
DOSE-RESPONSE RELATIONSHIPS
INOSITOL
LIVER CELLS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
RATS
TRACER TECHNIQUES
ALCOHOLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLESTERASES
CARCINOGENS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
ESTERS
HYDROLASES
HYDROXY COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550201 - Biochemistry- Tracer Techniques