Determination of /sup 14/CO/sub 2/ in breath and /sup 14/C in stool after oral administration of cholyl-1-(/sup 14/C)glycine: clinical application

Journal Article · Tue Nov 01 00:00:00 EST 1977 · Clin. Chem.; (United States)

OSTI ID:5088302

Roda, A ^[1]; Roda, E; Aldini, R; Mazzella, G; Festi, D; Sama, C; Barbara, L

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1. Univ. of Bologna

Twenty patients with intestinal bacterial overgrowth and 20 control subjects were investigated for bile acid deconjugation, by measuring /sup 14/CO/sub 2/ in the breath after cholyl-1-(/sup 14/C)glycine administration. /sup 14/CO/sub 2/ output/24 h was 11.0 +- 5.2% (mean +- SD) in controls and 54.2 +- 14.0% (mean +- SD) in bacterial-overgrowth patients (P < 0.001). /sup 14/CO/sub 2/ excretion rate in 12 h, when normalized to 100% of the dose at the 12th hour, gave an even finer discrimination between the two groups (no false responses). /sup 14/C in stool, analyzed in 20 malabsorption patients and 20 controls by two different techniques, was 6.6 +- 4% and 31.38 +- 20.7% (mean +- SD), respectively. Results by the two different techniques described here correlated well (r = 0.99). Bile acid malabsorption was in reasonable agreement (r = 0.67) with percentage of ''chenoid'' (chenodeoxycholic acid plus ursodeoxycholic acid) in the stool by gas-liquid chromatography; a poorer correlation was observed when ''choloid'' (cholic acid plus its epimers) were plotted vs. /sup 14/C in stool (r = 0.57, n = 15).

• OSTI ID: 5088302
• Journal Information: Clin. Chem.; (United States), Vol. 23:11
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BREATH
CHEMICAL ANALYSIS
FECES
INTESTINES
BACTERIA
AMINO ACIDS
BILE ACIDS
CARBON 14
CARBON DIOXIDE
ORAL ADMINISTRATION
PATIENTS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
CARBON COMPOUNDS
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
DIGESTIVE SYSTEM
EVEN-EVEN NUCLEI
GASTROINTESTINAL TRACT
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADIOISOTOPES
STEROIDS
STEROLS
WASTES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics