Characterization and sequence analysis of the human homeobox-containing gene GBX2

Journal Article · Thu Feb 01 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0056· OSTI ID:508333

Lin, Xu; Vaccarino, F M; Haas, M ^[1]

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1. Yale Univ., New Haven, CT (United States); and others

Polymerase chain reaction (PCR) was used to amplify portions of homeobox genes present in a human 11-week fetal brain cDNA library. One of these PCR products was determined by sequencing to be the Gastrulation and brain specific-2 gene (GBX2). Screening this human fetal brain cDNA library with probes specific for GBX2 led to the identification of a 2151-bp clone encodes for a protein of 347 amino acid residues. The amino acid sequence of the GBX2 homeodomain is identical (100%) to the that of homologous gene, Gbx2, expressed in the developing mouse embryo and virtually identical (97%) to a gene expressed in the developing chicken embryo, CHox7. The 5{prime} end of the GBX2 gene contains a CpG island in the untranslated region and a trinucleotide (CCG){sub 8} repeat in the coding region. The amino-terminal end of the GBX2 protein is proline-rich, with 30 proline residues in one stretch of 120 by Northern analysis in the developing human CNS as well as in other tissues. The human genomic clone for GBX2 was also isolated, characterized, and mapped to 2q36(d)-q37 by somatic cell hybrid analysis and fluorescence in situ hybridization. These studies provide a framework for designing future experiments that are needed to determine the functional significance of this gene in CNS development. 38 refs., 4 figs.

• OSTI ID: 508333
• Journal Information: Genomics, Vol. 31, Issue 3; Other Information: PBD: 1 Feb 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOME 2
GENETIC MAPPING
GENES
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA-CLONING
GENE REGULATION
TISSUE DISTRIBUTION
TRANSCRIPTION
DNA SEQUENCING
MICE
ONTOGENESIS
CHICKENS
CENTRAL NERVOUS SYSTEM
NUCLEOTIDES
POLYMERASE CHAIN REACTION
SOMATIC CELLS
HYBRIDIZATION
DNA HYBRIDIZATION
FLUORESCENCE
PROBES
AMINO ACID SEQUENCE
PROLINE