Characterization of the human 5-lipoxygenase gene promoter

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.23.9073· OSTI ID:5080053

Hoshiko, S; Radmark, O; Samuelsson, B ^[1]

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1. Karolinska Inst., Stockholm (Sweden)

Nucleotide sequences that direct transcription of the human 5-lipoxygenase gene have been examined by ligation to the chloramphenicol acetyltransferase activity in transfected HeLa and HL-60 cells. Various lengths of 5{prime}-flanking sequences up to 5.9 kilobase pairs 5{prime} of the transcriptional initiation sites were tested. Two positive and two negative apparent regulatory regions were seen. Part of the promoter sequence ({minus}179 to {minus}56 from ATG), which includes five repeated GC boxes (the putative Spl binding sequence) was essential for transcription in both HeLa and HL-60 cells. Gel-shift assays (using the DNA fragment {minus}212 to {minus}88) revealed that the transcriptional factor Spl could bind to this region of the 5-lipoxygenase promoter. Furthermore, HL-60 nuclear extracts contained specific nuclear factor(s) binding to 5-lipoxygenase promoter DNA, which could not be detected in HeLa cell nuclear extracts.

• OSTI ID: 5080053
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:23; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REPRESSORS
DNA SEQUENCING
OXYGENASES
TRANSCRIPTION
CARBON 14 COMPOUNDS
CHLORAMPHENICOL
HELA CELLS
LUCIFERASE
MAN
MESSENGER-RNA
RECOMBINANT DNA
TRANSFERASES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CARBON COMPOUNDS
DNA
DRUGS
ENZYMES
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques