Different mRNAs code for dopa decarboxylase in tissues of neuronal and nonneuronal origin
- College de France, Paris (France)
A cDNA clone for dopa decarboxylase has been isolated from a rat pheochromocytoma cDNA library and the cDNA sequence has been determined. It corresponds to an mRNA of 2094 nucleotides. The length of the mRNA was measured by primer-extension of rat pheochromocytoma RNA and the 5{prime} end of the sequence of the mRNA was confirmed by the PCR. A probe spanning the translation initiation site of the mRNA was used to hybridize with mRNAs from various organs of the rat. S1 nuclease digestion of the mRNAs annealed with this probe revealed two classes of mRNAs. The comparison of the cDNA sequence and published sequences for rat liver, human pheochromocytoma, and Droxophila dopa decarboxylase supported the conclusion that two mRNAs are produced: one is specific for tissue of neuronal origin and the other is specific for tissues of nonneuronal (mesodermal or endodermal) origin. The neuronal mRNA contains a 5{prime} untranslated sequence that is highly conserved between human and rat pheochromocytoma including a GA stretch. The coding sequence and the 3{prime} untranslated sequence of mRNAs from rat liver and pheochromocytoma are identical. The rat mRNA differs only in the 5{prime} untranslated region. Thus a unique gene codes for dopa decarboxylase and this gene gives rise to at least two transcripts presumably in response to different signals during development.
- OSTI ID:
- 5079079
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:6; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DECARBOXYLASES
AMINO ACID SEQUENCE
MESSENGER-RNA
MOLECULAR STRUCTURE
RATS
DNA SEQUENCING
DOPA
DROSOPHILA
LIVER
NERVE CELLS
PHOSPHORUS 32
RECOMBINANT DNA
TUMOR CELLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ARTHROPODS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DIPTERA
DNA
DRUGS
ENZYMES
FLIES
FRUIT FLIES
GLANDS
HYDROXY ACIDS
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
LYASES
MAMMALS
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques