Activity ratios of ribulose-1,5-bisphosphate carboxylase accurately reflect carbamylation ratios. [Phaseolus vulgaris, Spinacla oleracea]

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.3.735· OSTI ID:5047072

Butz, N D; Sharkey, T D ^[1]

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1. Univ. of Wisconsin, Madison (USA)

Activity ratios and carbamylation ratios of ribulose-1,5-bisphosphate carboxylase (RuBPCase) were determined for leaves of Phaseolus vulgaris and Spinacia oleracea exposed to a variety of partial pressures of CO{sub 2} and O{sub 2} and photon flux densities (PFD). It was found that activity ratios accurately predicted carbamylation ratios except in extracts from leaves held in low PFD. In particular, it was confirmed that the loss of FuBPCase activity in low partial pressure of O{sub 2} and high PFD results from reduced carbamylation. Activity ratios of RuBPCase were lower than carbamylation ratios for Phaseolus leaves sampled in low PFD, presumably because of the presence of 2-carboxyarabinitol 1-phosphate. Spinacia leaves sampled in darkness also exhibited lower activity ratios than carbamylation ratios indicating that this species may also have an RuBPCase inhibitor even though carboxyarabinitol 1-phosphate has not been detected in this species in the past.

• OSTI ID: 5047072
• Journal Information: Plant Physiology; (USA), Vol. 89:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHASEOLUS
ENZYME ACTIVITY
SPINACH
CARBAMATES
CARBON DIOXIDE
CARBON DIOXIDE FIXATION
CARBOXYLATION
FLUX DENSITY
LEAVES
MAGNESIUM COMPOUNDS
OXYGEN
PARTIAL PRESSURE
RIBULOSE DIPHOSPHATE CARBOXYLASE
ALKALINE EARTH METAL COMPOUNDS
CARBON COMPOUNDS
CARBON OXIDES
CARBON-CARBON LYASES
CARBONIC ACID DERIVATIVES
CARBOXY-LYASES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
FOOD
LEGUMINOSAE
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
VEGETABLES
551000* - Physiological Systems