Aldosterone metabolism in the isolated perfused liver of female and male rats

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-125-6-3011· OSTI ID:5035814

Egfjord, M; Olgaard, K ^[1]

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1. Rigshospitalet, Copenhagen (Denmark)

A sex-dependent metabolism of aldosterone has been reported in intact rats. To further characterize the hepatic elimination of aldosterone and its sex dependence, the metabolism of d-(4-14C)aldosterone was studied in isolated perfused liver from male and female Wistar rats, from male rats castrated 3 weeks before experiments, and from younger male rats (same body weight as the female rats). The livers were perfused at a constant flow rate in a recirculating mode with a hemoglobin-free medium containing aldosterone at initially 1 nM. Perfusate aldosterone was measured by a specific RIA. Total 4-14C radio-activity in perfusate and bile was determined. The perfusate (4-14C)aldosterone radiometabolite concentration was calculated. The radiometabolite pattern in additional experiments was studied by HPLC. The male rats exhibited 10% higher systolic blood pressure (P less than 0.05) and 51% higher fasting values of plasma aldosterone (P less than 0.05) compared to those in the female rats. In female rats the hepatic clearance rate of aldosterone per 100 g BW was 72% higher than that in male rats (11.2 +/- 2.7 to 6.5 +/- 1.8 ml/min: P less than 0.01), and that expressed per g liver wet wt was 75% higher (3.5 +/- 1.0 to 2.0 +/- 0.7 ml/min; P less than 0.01). When female rats were compared to younger male rats with the same body weight, 33% higher hepatic aldosterone clearance rates were still found in female rats (21.0 +/- 5.4 to 15.8 +/- 3.2 ml/min; P less than 0.05), and 51% higher values when expressed per g liver wet wt (3.5 +/- 1.0 to 2.3 +/- 0.5 ml/min; P less than 0.01). No difference in the aldosterone clearance rate was observed in castrated male rats compared to that in noncastrated male rats. 4-14C-Labeled radiometabolite levels accumulated similarly in the perfusate of livers of both sexes.

• OSTI ID: 5035814
• Journal Information: Endocrinology; (USA), Vol. 125:6; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALDOSTERONE
METABOLISM
BILE
BIOLOGICAL HALF-LIFE
BLOOD PRESSURE
CARBON 14 COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
METABOLITES
PERFUSED ORGANS
RATS
SEX DEPENDENCE
TRACER TECHNIQUES
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHROMATOGRAPHY
CORTICOSTEROIDS
DIGESTIVE SYSTEM
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RODENTS
SEPARATION PROCESSES
STEROIDS
VERTEBRATES
550501* - Metabolism- Tracer Techniques