Evidence for mouse Th1- and Th2-like helper T cells in vivo. Selective reduction of Th1-like cells after total lymphoid irradiation
- Stanford Univ. School of Medicine, CA (USA)
Purified CD4+ BALB/c spleen T cells obtained 4-6 wk after total lymphoid irradiation (TLI) helped normal syngeneic B cells to produce a vigorous antibody response to TNP keyhole limpet hemocyanin in adoptive cell transfer experiments. However, the same cells failed to transfer delayed-type hypersensitivity to the adoptive hosts as measured by a foot pad swelling assay. In addition, purified CD4+ cells from TLI-treated mice were unable to induce graft vs. host disease in lethally irradiated allogeneic C57BL/Ka recipient mice. In response to mitogen stimulation, unfractionated spleen cells obtained from TLI mice secreted normal levels of IL-4 and IL-5, but markedly reduced levels of IL-2 and INF-gamma. A total of 229 CD4+ clones from spleen cells of both normal and TLI-treated mice were established, and the cytokine secretion pattern from each clone was analyzed. The results demonstrate that the ratio of Th1- and Th2-like clones in the spleens of normal BALB/c mice is 1:0.6, whereas the ratio in TLI mice is approximately 1:7. These results suggest that Th2-like cells recover rapidly (at approximately 4-6 wk) after TLI treatment and account for the early return of antibody helper activity and secretion of IL-4 and IL-5, but Th1-like cells recover more slowly (in approximately 3 mo) after irradiation, and this accounts for the deficit in cell-mediated immunity and the reduced amount of IL-2 and IFN-gamma secretion.
- OSTI ID:
- 5024709
- Journal Information:
- Journal of Experimental Medicine; (USA), Vol. 170:5; ISSN 0022-1007
- Country of Publication:
- United States
- Language:
- English
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IMMUNITY
RADIOINDUCTION
LYMPHOCYTES
BIOLOGICAL FUNCTIONS
LYMPHOKINES
SECRETION
ANTIBODY FORMATION
CLONE CELLS
GRAFT-HOST REACTION
INTERFERON
LETHAL IRRADIATION
MICE
SPLEEN CELLS
TRANSPLANTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
FUNCTIONS
GROWTH FACTORS
IRRADIATION
LEUKOCYTES
MAMMALS
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MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
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560152* - Radiation Effects on Animals- Animals