Multinuclear NMR studies of the formation of platinum(II)-adenine nucleotide complexes
There has been considerable interest in the mode of interaction of the simple antitumor agent cis-platin (cis-dichlorodiammineplatinum(II)) with nucleic acids. The authors have recently shown that Pt(II) has a high affinity for inorganic phosphate and polyphosphate ligands, and forms a variety of species that have been characterized. Phosphorus-31, carbon-13, and proton NMR studies of the adenine nucleotide complexes of Pt(II) have shown that the phosphate groups of these ligands do play a role in metal ion binding, in contrast to previous work that had indicated no interaction of the phosphate oxygens with PT(II). Kinetic studies have indicated that Pt(II) binding is a two-step process in which the initial complex that is formed is slowly converted to the final products. NMR studies have suggested that a phosphate-bound intermediate is formed initially, since coordination shifts due to complex formation are observed in the /sup 31/P spectra at an early stage in the reaction time course, while shifts are observed considerably later in the proton spectra. At least two final products are observed in the binding of Pt(II) to either AMP, ADP, or ATP. All of the final Pt(II) nucleotide complexes appear to involve interactions with the phosphate groups.
- Research Organization:
- Univ. of Akron, OH
- OSTI ID:
- 5020216
- Report Number(s):
- CONF-8606151-; TRN: 86-034835
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
PLATINUM
BIOCHEMICAL REACTION KINETICS
ADENINES
CARBON 13
LIGANDS
NMR SPECTRA
NUCLEOTIDES
PHOSPHATES
PHOSPHORUS 31
PROTONS
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BARYONS
CARBON ISOTOPES
DRUGS
ELEMENTARY PARTICLES
ELEMENTS
EVEN-ODD NUCLEI
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
METALS
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLATINUM METALS
PURINES
REACTION KINETICS
SPECTRA
STABLE ISOTOPES
TRANSITION ELEMENTS
550201* - Biochemistry- Tracer Techniques
550600 - Medicine