Characterization of arsenic compounds formed by Daphnia magna and Tetraselmis chuii from inorganic arsenate
Experiments to grow Tetraselmis chuii (a marine alga) and Daphnia magna in the presence of inorganic arsenate are described. The algae incorporate arsenic rather efficiently and form a lipid-soluble organic arsenic compound, T. chuii has been successfully mass cultured in a medium containing 10 ppm arsenic as arsenate. Daphnia magna was cultured in a medium containing /sup 74/As-labeled H/sub 3/AsO/sub 4/ and 1 ppm Na/sub 2/HAsO/sub 4/ expressed as arsenic. The arsenic metabolites were extracted with a chloroform-methanol solution and isolated by using column and thin-layer chromatography. TLC analysis of the metabolites revealed the presence of a /sup 74/As-containing product which migrated with phosphatidylethanolamine. This product was hydrolyzed with the phospholipases A, C, and D. The experimental results are not inconsistent with the presence of an arsenocholine moiety in the lipids. Arsenocholine, arsenobetaine, and acetylarsenocholine have been synthesized and will serve as reference substances in the chromatography experiments. The preparation of arsenocholine-containing lipids is in progress.
- Research Organization:
- Texas A and M, College Station
- OSTI ID:
- 5018517
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 19
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALGAE
CELL PROLIFERATION
ARSENATES
METABOLISM
ARSENIC COMPOUNDS
BIOCHEMISTRY
EXPERIMENTAL DATA
LIPIDS
CHEMISTRY
DATA
INFORMATION
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)