Freeze-drying and related preparation techniques for biological microprobe analysis
An X-ray microanalytical and morphological investigation has been carried out on rapidly frozen, freeze-dried or freeze-substituted tissues. A comparison was made between different embedding and polymerization procedures following freeze-substitution and freeze-drying. The investigation also included an analysis of specimens infiltrated, embedded and polymerized by ultraviolet irradiation at low temperatures with Lowicryl HM20. The morphological preservation of Lowicryl embedded tissue was adequate for the identification of different cell structures like nuclei, mitochondria, lysosomes and different types of endoplasmic reticulum. X-ray microanalytical investigation of low temperature embedded material displayed an elemental composition of cells and organelles similar to that found in freeze-dried cyosections. Compared with freeze-dried cryosections, low temperature embedded material could be sectioned for light microscopy and area of interest chosen for further thin sectioning. This is of great importance in work with tissues with complicated morphology and heterogenous cell populations.
- OSTI ID:
- 5016588
- Journal Information:
- Scanning Electron Microsc.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
ELECTRON MICROPROBE ANALYSIS
SAMPLE PREPARATION
CELL NUCLEI
COMPARATIVE EVALUATIONS
ENDOPLASMIC RETICULUM
FREEZING
HISTOLOGICAL TECHNIQUES
LIVER
LYOPHILIZATION
MITOCHONDRIA
MUSCLES
POLYMERS
RATS
ULTRAVIOLET RADIATION
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
GLANDS
MAMMALS
MICROANALYSIS
ORGANOIDS
ORGANS
RADIATIONS
RODENTS
VERTEBRATES
550301* - Cytology- Tracer Techniques