Cadmium-induced lung injury: cell kinetics and long-term effects
The effects of exposure to CdCl/sub 2/ aerosols followed by hyperoxia were studied in mouse lung. Special emphasis was placed on analysis of cell proliferation following injury. Male Balb/c mice were exposed to aerosols of 4.9 micrograms Cd/liter for 1 hr while controls were exposed to water aerosols. Immediately after, half of each group was placed in 80% O/sub 2/ for 6 days, while the rest were left in room air. Three endpoints were used to assess lung injury; measurement of hydroxyproline, (/sup 14/C)thymidine incorporation into DNA, and histopathology. Parenchymal and bronchiolar labeling indices were determined following autoradiography. A 1-hr exposure to CdCl/sub 2/ aerosols caused marked cell proliferation in the lung with the peak of cell labeling occurring at Day 5. In animals exposed to both CdCl/sub 2/ + 80% O/sub 2/, the cell labeling peak was delayed until Day 9. Cell differentiation studies showed a delay in the peak of type II epithelial cell and endothelial cell division when CdCl/sub 2/ exposure was followed by the 80% O/sub 2/ treatment. On Day 15 most of the labeled cells were identified as interstitial cells in both treated groups. Bronchiolar cell labeling was suppressed at the early time period in the Cd + O/sub 2/ group. With time, the histologically visible lung lesions tended to resolve in animals exposed to CdCl/sub 2/ or CdCl/sub 2/ and 80% O/sub 2/, whereas total pulmonary hydroxyproline remained at all times (3, 6, and 12 months) significantly higher in Cd-treated animals when compared to controls. It was concluded that acute lung injury by a toxic inhalant can be amplified if there is an initial delay in pulmonary cell proliferation following an acute insult.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5014259
- Journal Information:
- Toxicol. Appl. Pharmacol.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Pathogenesis and modification of cadmium-induced lung injury in mice
Respiratory toxicology following inhalation of NO/sub x/
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CADMIUM
TOXICITY
LUNGS
PATHOLOGICAL CHANGES
AEROSOLS
AUTORADIOGRAPHY
BRONCHI
CARBON 14 COMPOUNDS
CELL DIVISION
CELL PROLIFERATION
DNA
ENDOTHELIUM
EPITHELIUM
HYDROXYPROLINE
INHALATION
MICE
OXYGEN
THYMIDINE
TRACER TECHNIQUES
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZINES
AZOLES
BODY
CARBOXYLIC ACIDS
COLLOIDS
DISPERSIONS
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
NONMETALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
PYRROLES
PYRROLIDINES
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
SOLS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550201 - Biochemistry- Tracer Techniques