Effects of storage conditions on bovine carrier erythrocytes

Journal Article · Wed May 01 00:00:00 EDT 1985 · Am. J. Vet. Res.; (United States)

OSTI ID:5012809

DeLoach, J R

Bovine carrier RBC containing (/sup 14/C)sucrose as a marker were stored for 23 days in protein-free media. Cell recovery was highest with a phosphate buffer (pH 7.0) containing 2 mM MgCl/sub 2/, 5 mM inosine, 5 mM adenosine, and 10 mM glucose. A cell recovery of 60% was observed after 23 days of storage at 4 C. Stored carrier erythrocytes reinjected into cattle had an 18-day half-life, which is less than that for fresh carrier RBC.

• OSTI ID: 5012809
• Journal Information: Am. J. Vet. Res.; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ERYTHROCYTES
BIOLOGICAL RECOVERY
STORAGE
ADENINES
BIOLOGICAL HALF-LIFE
BUFFERS
CARBON 14 COMPOUNDS
CATTLE
GLUCOSE
INOSINE
SACCHAROSE
TRACER TECHNIQUES
ALDEHYDES
AMINES
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
DISACCHARIDES
DOMESTIC ANIMALS
DRUGS
HETEROCYCLIC COMPOUNDS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RECOVERY
RIBOSIDES
RUMINANTS
SACCHARIDES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques