Normal human neutrophils are a source of a specific interleukin 1 inhibitor
In the course of the study on neutrophil production of an interleukin 1 (IL 1)-like factor, it was found that the addition of polymorphonuclear neutrophils (PMN) to monocytes cultured in the presence of zymosan resulted in decreased IL 1 activity of the resultant supernatant, suggesting that PMN may contain an inhibitor of IL 1. The objective of this investigation was to study this IL 1 inhibitor which normal human PMN contain. The inhibitor is constitutively present in the PMN because 0 hr PMN lysates and unstimulated PMN supernatants also show inhibitory activity. The PMN inhibitor inhibits IL 1 (crude and partially purified) in a dose-response manner and does not affect basal (/sup 3/H)thymidine incorporation in the presence or absence of PHA-P. The PMN inhibitor does not have any effect on interleukin 2 (IL 2)-induced proliferation of the IL 2-dependent CTLL cells. The inhibitor can be generated in the absence of serum and is not produced as a result of proteolytic activity from PMN enzymes. The inhibitor is heat-labile and is most stable at neutral pH. Gel filtration studies on Sephadex G-200 indicate that the inhibitor is heterogeneous in size. Two inhibitory peaks, at 45,000 to 70,000 m.w. and at greater than 160,000 m.w., were observed. When zymosan-stimulated PMN supernatant was chromatographed, there was separation of inhibitory factor from a 17,000 m.w. proliferating factor. Presence of this PMN inhibitor may be important in negative regulation of IL 1.
- Research Organization:
- Univ. of Illinois, Chicago
- OSTI ID:
- 5009189
- Journal Information:
- J. Immunol.; (United States), Vol. 136:10
- Country of Publication:
- United States
- Language:
- English
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BIOCHEMICAL REACTION KINETICS
INHIBITION
CELL PROLIFERATION
DOSE-RESPONSE RELATIONSHIPS
MONOCYTES
NEUTROPHILS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
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BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
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MATERIALS
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PROTEINS
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550201* - Biochemistry- Tracer Techniques