16S rDNA-based probes for two polycyclic aromatic hydrocarbon (PAH)-degrading soil Mycobacteria
- Univ. of Cincinnati Medical Center, OH (United States)
PAHs are a class of widespread pollutants, some of which have been shown to be genotoxic, hence the fate of these compounds in the environment is of considerable interest. Research on the biodegradation of 4 and 5 ring PAHs has been limited by the general lack of microbial isolates or consortia which can completely degrade these toxicants. Heitkamp and Cerniglia have described an oxidative soil Mycobacterium-strain PYR-1 that metabolizes pyrene and fluoranthene more rapidly than the 2 and 3 ring naphthalene and phenanthrene; although some metabolites of benzo-(a)-pyrene (BaP) were detected, no mineralization of BaP was observed. In 1991 Grosser et al. reported the isolation of a Mycobacterium sp. which mineralizes pyrene and also causing some mineralization of BaP. Their study describes a comparative analysis of these two strains, which show very similar colony morphology, growth rate and yellow-orange pigmentation. Genetic differences were shown by DNA amplification fingerprinting (DAF) using two arbitrary GC-rich octanucleotide primers, and by sequence comparison of PCR amplified 16S rDNA, although both strains show similarity closest to that of the genus Mycobacteria. These 16S rDNA sequences are in use for the construction of strain-specific DNA probes to monitor the presence, survival and growth of these isolates in PAH-contaminated soils in studies of biodegradation.
- Sponsoring Organization:
- National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
- OSTI ID:
- 491064
- Report Number(s):
- CONF-9406250-; TRN: IM9729%%315
- Resource Relation:
- Conference: 9. Annual conference on hazardous waste remediation, Bozeman, MT (United States), 8-10 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Proceedings of the 9. annual conference on hazardous waste remediation; Erickson, L.E.; Tillison, D.L.; Grant, S.C.; McDonald, J.P. [eds.]; PB: 389 p.
- Country of Publication:
- United States
- Language:
- English
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