Studies in applying Pseudomonas cepacia PR1-(pTOM{sub 31C}) to a TCE bioreactor
- Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering
- Univ. of West Florida, Pensacola, FL (United States). Center for Diagnostics and Bioremediation
The pTOM{sub 31C} plasmid was derived from the large plasmid of Pseudomonas cepacia G4. Strain G4 is an environmental isolate that is capable of cometabolic mineralization of trichloroethylene (TCE). The degradation of TCE by strain G4 is a result of the induction of enzymes in a non-constitutive aromatic degradative pathway that is present on the large plasmid of G4. A Tn5 insertion into the large plasmid of G4 resulted in a TCE constitutive plasmid designated as pTOM{sub 31C}. A number of laboratory and field studies have been performed to determine the feasibility of applying pTOM{sub 31C}, via a host microbe designated Ps. cepacia PR1-(pTOM{sub 31C}), into vapor phase TCE bioreactors. Results from degradation studies show a linear degradation rate of TCE by PR1-(pTOM{sub 31C}). The growth kinetics of PR1-(pTOM{sub 31C}) on phthalate followed the Monod kinetics model. However, bench scale studies showed that PR1-(pTOM{sub 31C}) growing on phthalate was incapable of developing a substantial biofilm. The results from a number of bench scale and field scale reactor studies showed that PR1-(pTOM{sub 31}) could not compete with invading microorganisms, even when selective pressures for PR1 were applied to the systems. In the field scale reactors, PR1-(pTOM{sub 31C}) was able to colonize only the top fraction of the oyster shell support media.
- OSTI ID:
- 491020
- Report Number(s):
- CONF-9406250-; TRN: IM9729%%271
- Resource Relation:
- Conference: 9. Annual conference on hazardous waste remediation, Bozeman, MT (United States), 8-10 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Proceedings of the 9. annual conference on hazardous waste remediation; Erickson, L.E.; Tillison, D.L.; Grant, S.C.; McDonald, J.P. [eds.]; PB: 389 p.
- Country of Publication:
- United States
- Language:
- English
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