Localization of eight additional genes in the human major histocompatibility complex, including the gene encoding the casein kinase II {beta} subunit (CSNK2B)

Journal Article · Sun Sep 01 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0459· OSTI ID:486309

Albertella, M R; Jones, H; Thomson, W ^[1]

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1. Oxford Univ. (United Kingdom); and others

A wide range of autoimmune and other diseases are known to be associated with the major histocompatibility complex. Many of these diseases are linked to the genes encoding the polymorphic histocompatibility complex. Many of these diseases are linked to the genes encoding the polymorphic histocompatibility antigens in the class I and class II regions, but some appear to be more strongly associated with genes in the central 1100-kb class III region, making it important to characterize this region fully for the presence of novel genes. An {approximately}220-kb segment of DNA in the class III region separating the Hsp70 (HSPA1L) and BAT1 (D6S8IE) genes, which was previously known to contain 14 genes. Genomic DNA fragments spanning the gaps between the known genes were used as probes to isolate cDNAs corresponding to five new genes within this region. Evidence from Northern blot analysis and exon trapping experiments that suggested the presence of at least two more new genes was also obtained. Partial cDNA and complete exonic genomic sequencing of one of the new genes has identified it as the casein kinase II{beta} subunit (CSNK2B). Two of the other novel genes lie within a region syntenic to that implicated in susceptibility to experimental allergic orchitis in the mouse, an autoimmune disease of the testis, and represent additional candidates for the Orch-1 locus associated with this disease. In addition, characterization of the 13-kb intergenic gap separating the RD (D6545) and G11 (D6S60E) genes has revealed the presence of a gene encoding a 1246-amino-acid polypeptide that shows significant sequence similarity to the yeast anti-viral Ski2p gene product. 49 refs., 8 figs.

• OSTI ID: 486309
• Journal Information: Genomics, Vol. 36, Issue 2; Other Information: PBD: 1 Sep 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
DNA SEQUENCING
GENETIC MAPPING
TRANSCRIPTION
HUMAN CHROMOSOMES
HISTOCOMPATIBILITY COMPLEX
HEREDITARY DISEASES
GENETICS
RISK ASSESSMENT
MAN
IMMUNE SYSTEM DISEASES
PHOSPHOTRANSFERASES
EXONS
AMINO ACID SEQUENCE
CASEIN
ANTIGENS
POLYPEPTIDES
COSMIDS
CONTIGS
DNA HYBRIDIZATION
PROBES
POLYMERASE CHAIN REACTION
NUCLEOTIDES