Genomic DNA sequence, expression, and chromosomal localization of the human B1 bradykinin receptor gene BDKRB1

Journal Article · Mon Jan 01 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0008· OSTI ID:478833

Chai, K X; Chao, J; Chao, L ^[1]

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1. Medical Univ. of South Carolina, Charleston, SC (United States); and others

We have cloned and sequenced the human B1 bradykinin receptor gene (BDKRB1), which contains as uninterrupted coding exon. A putative promoter was identified by linking various lengths of the 5{prime}-flanking region of the B1 receptor gene coding sequence to a CAT reporter and assaying for CAT activity. Deletion analysis showed that a 300-bp fragment in the promoter region is sufficient to direct the synthesis of the reporter and that an enhancer-like element is present between -1842 and -812. A genomic Southern blot using the B1 cDNA revealed that the receptor is encoded by a single-copy gene. The gene is located on chromosome 14q32.1-32.2, in close proximity to the B2 receptor gene. Northern blot analysis identified a 1.7- to 1.8-kb mature mRNA transcript of the B1 receptor gene in the kidney and pancreas. A widespread tissue distribution of the B1 gene expression was identified by RT-PCR-Southern blot analysis using specific oligonucleotide probes. 25 refs., 6 figs.

• OSTI ID: 478833
• Journal Information: Genomics, Vol. 31, Issue 1; Other Information: PBD: 1 Jan 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
HUMAN CHROMOSOME 14
GENETIC MAPPING
RECEPTORS
TRANSCRIPTION
TISSUE DISTRIBUTION
DNA-CLONING
DNA SEQUENCING
GENE REGULATION
STRUCTURE-ACTIVITY RELATIONSHIPS
BRADYKININ
HEREDITARY DISEASES
PATIENTS
GENES
OLIGONUCLEOTIDES
BIOASSAY
POLYMERASE CHAIN REACTION
PROBES
DNA HYBRIDIZATION