Three methods for large-scale preservation of a microbial inoculum for bioremediation
- BioRenewal Technologies, Inc., Madison, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical Engineering
Spray-drying, freeze-drying, and freezing were evaluated as methods of preserving Alcaligenes eutrophus H850 cultures for use as a microbial inoculum in bioremediation. A. eutrophus was cultivated on succinate or biphenyl prior to spray-drying, freeze-drying, or freezing. Dehydration inactivation was the primary reason for the loss of viability and degradation activity after spray-drying and freeze-drying. Thermal inactivation played a substantially lesser role. The ability of surviving bacteria to grow on biphenyl was more sensitive to drying than was overall survival of the microorganisms. Of the three preservation methods evaluated, freezing produced cultures with substantially higher survival and ability to grow on biphenyl than did freeze-drying or spray-drying.
- OSTI ID:
- 474254
- Report Number(s):
- CONF-950483-; ISBN 1-57477-004-7; TRN: IM9723%%144
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioaugmentation for site remediation; Hinchee, R.E.; Fredrickson, J.; Alleman, B.C. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; PB: 272 p.; Bioremediation, Volume 3(3)
- Country of Publication:
- United States
- Language:
- English
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