PROTECTION OF TISSUE-CULTURE CELLS AGAINST IONIZING RADIATION. II. THE ACTIVITY OF HYPOXIA, DIMETHYL SULPHOXIDE, DIMETHYL SULPHONE, GLYCEROL AND CYSTEAMINE AT ROOM TEMPERATURE AND AT -196 C
Protection against x irradiation by hypoxia, glycerol, dimethyl sulfoxide, dimethyl sulfone, cystearnine, and a combination of glycerol and cysteamine was investigated at +20 deg C and -198 deg C. The protective power of the treatment was assayed as the degree to which it prevented radiation damage, i.e., the loss of the reproductive integrity of tissue-culture cells. Using cells attached to a Melinex sheet the following dose-reduction factors (DRF) were found: 15 volume per cent glycerol, 1.9; 15 per cent glycerol + 4 mm cysteamine, 2.8; 0.5, 1, 2, 4, 8, 8, 15, and 30 volume per cent dimethyl sulfoxide, 1.13, 1.3, 1.38, 1.65, and anoxia, 2.6. Dimethyl sulfone did not protect against irradiation. Cells in suspension were protected against freezing and thawing by glycerol and dimethyl sulfoxide. Dimethyl sulfone showed no protective activity. Freezing to a temperature of -198 deg C made cells in suspension less sensitive to x irradiation (DRF 1.9 to 2.8). In these conditions 15 volume per cent giycerol, 1 and 4 volume per cent dimethyl sulfoxide and a combination of 15 per cent glycerol and 4 mm cysteamine gave the same DRF at room temperature and at - 198 deg C (if survival was compared with controls irradiated at the same temperature). However, if for hypoxia similar calculations were made, a much better protection was found at room temperature than at -198 deg C (DRF 2.2 and 1.1). (auth)
- Research Organization:
- Toegepast Natuurwetenschappelijk Onderzoek, Rijswijk, Netherlands
- NSA Number:
- NSA-17-010493
- OSTI ID:
- 4710768
- Journal Information:
- Intern. J. Radiation Biol., Vol. Vol: 5; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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