AUTOMATIC AMINO ACID ANALYZER AS A NEW TOOL IN RADIATION RESEARCH (in Japanese)
A new automatic amino aeid analyzer with three new quantitative micro- pumps, a new three-wavelength photometer, and a programming system was used for amino acid analysls. Twenty amino acids were gamma irradiated both in dry powder forms and in aqueous solution. Survival amino acids were determined automatically by the automatic and no acids analyzer. The radiosensitivity of tyrosine, tryptophan, and cystine was characteristic under the conditions. A specimen of crystalline bacterial proteinase prepared from Baciilus subtilis was also exposed to gamma irradiation under the same conditions, and the proteolytic activity and amino acid composition were measured. As a result, it was found that about 50% of the full activity of the enzyme was left on the damaged enzyme protein from which all the tyrosine had disappeared. This suggests that there must be a conformatlonal change of active structure of the enzyme rather than a decomposition of amino acid structure of the protein. Some in vivo experiments on amino acid determinations in urine of rabbits after wholebody irradiation are also reported. Analyses were carried out successfully for the determination of cysteic acid, taurine, urea, aspartic acid, threonine, serine (plus glutannine and/or asparagine), glutamic acid, citrulline, proline, glycine, alanine, valine, cystathionine, methionine, isoleucine, leucine, tyrosine, phenylalanine, alpha -amino-isobutyric acid, tryptophan (plus creatinine), lysine (plus ornithine), histidine, 1- and 3-methylhistidine, ethanolamine, ammonia, arginine, and nine other uncharacterized components. (P.C.H.)
- Research Organization:
- Kyoto Univ.
- NSA Number:
- NSA-17-030214
- OSTI ID:
- 4707396
- Journal Information:
- Proc. Japan Conf. Radioisotopes, Vol. Vol: 5th, No. 4; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- Japanese
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Related Subjects
ALANINE
AMINES
AMINO ACIDS
AMMONIA
ARGININE
ASPARAGINE
ASPARTIC ACID
BACILLUS SUBTILIS
BACTERIA
CITRULLINE
CONFIGURATION
CREATININE
CRYSTALS
CYSTINE
DECOMPOSITION
ENZYMES
ETHANOL
FREQUENCY
GAMMA RADIATION
GAMMA SOURCES
GLUTAMIC ACID
GLUTAMINE
GLYCINE
HISTIDINE
IN VIVO
INSTRUMENTS
IRRADIATION
ISOBUTYRIC ACID
ISOLEUCINE
LABORATORY EQUIPMENT
LEUCINE
LYSINE
METHIONINE
METHYL RADICALS
ORGANIC ACIDS
ORGANIC NITROGEN COMPOUNDS
ORNITHINE
PHENYL RADICALS
PHOTOMETRY
PLANNING
POWDERS
PROLINE
PROTEINS
PUMPS
QUALITATIVE ANALYSIS
RABBITS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOSENSITIVITY
SERINE
SOLUTION