Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose: Ceramide galactosyltransferase gene

Journal Article · Mon Jul 01 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0341· OSTI ID:466037

Coetzee, T; Fujita, N; Marcus, J ^[1]

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1. Univ. of North Carolina, Chapel Hill, NC (United States); and others

UDP-galactose:ceramide galactosyltransferase (CGT) (EC 2.11.62) catalyzes the final step in the synthesis of galactocerebroside, a glycosphingolipid characteristically abundant in myelin. In this report, we describe the isolation of genomic clones spanning the mouse CGT gene. The mouse CGT gene consists of six exons that span a minimum of 70 kb of DNA and that encode a 541 amino acid translation product with extensive sequence similarity to the rat CGT enzyme and to UDP-glucuronosyltransferases (UGT). The 5{prime}-untranslated region of the mouse CGT gene is encoded by a separate exon located approximately 25 kb upstream of the first protein-encoding exon. Furthermore, the genomic organization of the five encoding region exons of the mouse CGT gene resembles that of the human UGT1 and rat UGT2B1 genes. Finally, analysis of somatic cell hybrids by PCR and fluorescence in situ hybridization to metaphase chromosomes has localized the mouse CGT gene to chromosome 3, bands E3-F1. 26 refs., 5 figs., 1 tab.

• OSTI ID: 466037
• Journal Information: Genomics, Vol. 35, Issue 1; Other Information: PBD: 1 Jul 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMES
GENETIC MAPPING
MICE
MUTANTS
HEXOSYL TRANSFERASES
DNA-CLONING
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
BIOLOGICAL EVOLUTION
TRANSCRIPTION
E CODES
CEREBROSIDES
BIOSYNTHESIS
NUCLEOTIDES
EXONS
AMINO ACID SEQUENCE
SOMATIC CELLS
HYBRIDIZATION
POLYMERASE CHAIN REACTION
IN-SITU HYBRIDIZATION
FLUORESCENCE
MYELIN