Rapid collection, detection, and assessment of environmental petroleum spills
- National Biological Service, Columbia, MO (United States)
The bioavailability and toxicity of petroleum oil spills were determined by a new tandem monitoring approach with a passive sorptive device for collection and concentration, and luminescent bacteria for detection. Crude oil, fuel oil, jet fuel, gasoline, and recycled motor oil, as model contaminants, were collected over eight weeks from sediment:water microcosms simulating environmental petroleum spills. Bioremediation strategies for removal of oil contaminants with organic and inorganic nutrients, heterotrophic bacterial strains, and detergent were monitored for efficacy. Air and waterborne oil contaminants was collected with a semipermeable membrane device (SPMD) that contains a thin film of triolein. Analytes were extracted from SPMDs by either dialysis or diluted with organic solvents. Acute toxicity and genotoxicity of analytes were determined with the luminescent bacterial assays Microtox{reg_sign} and Mutatox{reg_sign}. Changes in the bioavailability and toxicity of different oil products as well as influences of different bioremediation treatments were successfully monitored in aquatic microcosms over time. SPMDs and the Microtox-Mutatox systems used in combination offered a sensitive, rapid and cost-effective toxicological screening tool to assess the bioavailability of hazardous environmental oil contaminants.
- OSTI ID:
- 452079
- Report Number(s):
- CONF-961149-; TRN: 97:006626
- Resource Relation:
- Conference: 17. annual meeting of the Society of Environmental Toxicology and Chemistry: partnerships for the environment - science, education, and policy, Washington, DC (United States), 17-21 Nov 1996; Other Information: PBD: 1995; Related Information: Is Part Of SETAC 17. annual meeting -- Abstract book. Partnerships for the environment: Science, education, and policy; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
Similar Records
Rapid toxicity assessment of sediments from estuarine ecosystems: A new tandem in vitro testing approach
Monitoring of PCDDs, PCDFs and PCBs in the Fraser River, British Columbia using SPMD