Thermal inactivation of untreated and gamma-irradiated A2/Aichi/2/68 influenza virus
The infectivity and the hemagglutinin activily of A2/Aichi/2/68 influenza virus were unchanged during a 2-year storage in allantoic fluid at -8O deg C. Over the temperature range from -20 to +30 deg C, cxponential slopes could be drawn by means of the regression analysis, the velocity constants showing very low values. Conversely, at +56 deg C inactivation took place in a two-component fashion, each following first-order kinetics. Hemagglutinin and neuraminidase activities were not impaired by exposure to STA/sup 60/Co! gamma rays (3 x 10/ sup 6/ rad), which completely removed infectious particles. The specific rate constants for inactivation of hemagglutinin and neuraminidase activities of untreated and irradiated virus were overlapping when samples were stored for 2 yrs in the frozen state (-80 and -2O deg C), whereas a significantly increasing rate of inactivation was recorded for gamma -irradiated samples following storage at temperature above 4 deg C. Nevertheless, the energy of activation required for thermal inactivation was very low and the entropy of activation showed negative values for both the untreated and the irradiated virus preparations. (auth)
- Research Organization:
- Univ. of Genoa (Italy)
- NSA Number:
- NSA-29-002851
- OSTI ID:
- 4405826
- Journal Information:
- J. Gen. Virol., v. 20, no. 2, pp. 261-265, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: UK (United Kingdom (sent to DOE from))
- Country of Publication:
- United Kingdom
- Language:
- English
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