Studies on lysosomes after irradiation. II. Lysosomal membrane permeability and acid phosphatase activity of lymphoid and other tissues after whole-body irradiation
Lysosomal membrane permeability and acid phosphatase activity were studied histochemically in sections of tissues from mice which had been subjected to 50 to 1000 rads whole-body irradiation. After a whole-body dose of 850 rads, no change in lysosomal membrane permeability or of enzyme activity was observed in liver, kidney, heart, or adrenal, but substantial increases of membrane permeability and acid phosphatase activity were observed in spleen and thymus. A dose of 100 rads caused an increase in the permeability of lysosomal membranes in the spleen and alter 300 rads the membranes were fully permeable. Enzyme activity was increased, progressively, by doses up to 850 rads and even greater activation of enzyme was observed in the thymus. One hour after 850 rads, enzyme activity in spleen was significantly greater than controls, and it increased to a maximum 48 hr after irradiation. Full recovery of normal membrane permeability and enzyme activity occurred after lower doses. Increased enzyme activity is not considered to be, primarily, a consequence of increased enzyme synthesis or an artifact caused by changes in cell population. Injections of cortisone, hydrocortisone, or chlorambucil cause extensive lymphocytolysis and increase membrane permeability and enzyme activity. Lymphocytes damaged by irradiation are thus believed to release factors which cause increased permeability of the lysosomal membrane and increased acid phosphatase activity. (auth)
- Research Organization:
- St. Bartholomew's Hospital, London
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-29-026966
- OSTI ID:
- 4328148
- Journal Information:
- Radiat. Res., v. 57, no. 3, pp. 416-430, Other Information: Orig. Receipt Date: 30-JUN-74
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
*ACID PHOSPHATASE- BIOCHEMICAL REACTION KINETICS
*LYSOSOMES- PERMEABILITY
*SPLEEN- BIOLOGICAL RADIATION EFFECTS
*THYMUS- BIOLOGICAL RADIATION EFFECTS
ADRENAL GLANDS
ALKYLATING AGENTS
CATALYSIS
CORTISONE
DOSE- RESPONSE RELATIONSHIPS
ENZYMES
GAMMA RADIATION
HEART
HYDROCORTISONE
KIDNEYS
LIVER
LYMPHOCYTES
MEMBRANES
METABOLISM
PHOTON BEAMS