Morphogenesis of bacteriophage phi29 of Bacillus subtilis: cleavage and assembly of the neck appendage protein
Each of the 12 neck appendages of the Bacillus subtilis bacteriophage phi 29 consists of a single protein molecular weight of about 75,000, and on the mature virion the appendages are assembled to the lower of two collars. The appendage protein is cleaved from a percursor protein, P(J), with a molecular weight of about 88,000. This cleavage is independent of neck assembly, occurring during infection by mutants that cannot synthesize the proteins of the upper and lower collars of the neck. The cleaved form of the appendage protein is efficiently complemented in vitro to particles lacking appendages. Thus, cleavage of the appendage precursor protein apparently does not occur in situ on the maturing virus. (auth)
- Research Organization:
- Univ. of Minnesota, Minneapolis
- NSA Number:
- NSA-33-005711
- OSTI ID:
- 4177274
- Journal Information:
- J. Virol., v. 16, no. 5, pp. 1282-1295, Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560131* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies
*BACTERIOPHAGES- MORPHOLOGICAL CHANGES
*PROTEINS- MOLECULAR WEIGHT
BACILLUS SUBTILIS
CLEAVAGE
ULTRAVIOLET RADIATION