Analysis of uridine incorporation in chicken embryo cells infected by vesicular stomatitis virus and its temperature-sensitive mutants: uridine transport

Journal Article · Wed Jan 01 00:00:00 EST 1975 · J. Virol., v. 15, no. 1, pp. 8-15

OSTI ID:4168378

Genty, N

The shut-off of RNA synthesis in chicken embryo cells, after infection with vesicular stomatitis virus, is partially due to a reduced capacity of the infected cells to transport uridine. Permeability to uridine decreases exponentially after infection. This loss of ability to transport uridine may be caused either by structural components of the input virions or may result from the expression of the viral gene products. In the latter case, only minor levels of viral transcription are sufficient to modify cellular permeability, since, even at low multiplicities, RNA$sup -$ temperature-sensitive (ts) mutants of vesicular stomatitis virus bring about a significant diminution of uridine incorporation in cells infected under nonpermissive conditions. Experiments with mutants of group III suggest that the M protein of the viral envelope may play a role in the sequence of events that modifies uridine transport. In addition to this cause of the diminution of incorporation of uridine by infected cells, another mechanism is noted which requires protein synthesis. (auth)

• Research Organization: Univ. of Paris, Orsay, France
• NSA Number: NSA-33-005722
• OSTI ID: 4168378
• Journal Information: J. Virol., v. 15, no. 1, pp. 8-15, Other Information: Orig. Receipt Date: 30-JUN-76
• Country of Publication: United States
• Language: English

Related Subjects

N48310* -Life Sciences-Radiation Effects on Microorganisms- -Basic Studies
560131* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies
*EMBRYONIC CELLS- METABOLISM
*RNA- BIOSYNTHESIS
*URIDINE- DIFFUSION
*VIRUSES- BIOLOGICAL RADIATION EFFECTS
MUTANTS
PERMEABILITY
TEMPERATURE DEPENDENCE
ULTRAVIOLET RADIATION