THE EFFECT OF LANTHANIDES AND ACTINIDES ON BLOOD COAGULATION. I. EVIDENCE FOR AND PROPERTIES OF A NEW SERUM THROMBOPLASTIC FACTOR

Journal Article · Wed Apr 01 00:00:00 EST 1964 · Journal of Clinical Investigation (U.S.)

DOI:https://doi.org/10.1172/JCI104955· OSTI ID:4025772

Colman, R; Alexander, B

Thorotrast in vivo promptly induces retarded coagulation and in vitro a profound inability of both plasma and serum to support thromboplastin generation. The same abnormality is induced in seram by thorium chloride and the related elements, Nd, La, Ce, and U. The plasma defect consists of progressive inactivation of accelerator globulin (Factor V). The serum defect involves an entity distinct from any hithento known clotting factor or intermediate. It is adsorbable by BaSO/sub 4/, elutable by citrate, and it is demonstrable in the protein fraction precipitated at 50 to 60% (NH/sub 4/)/sub 2/SO/sub 4/. The vulnerable factor in serum (TVF) is relatively labi1e, was separated from the other coagulation factors by physicochemical means, and can be markedly activated by trypsin. Replacing Inosithin by normal platelets in the thromboplastin generation mixture corrects the Th-induced defect. In contrast, Th-treated platelets are inert. Citrate and oxalate completely reverse the Th-induced defect, but EDTA is ineffective. It is suggested that the lanthanldes and actinldes produce a defect in serum by forming a reversible complex with a serum protein, which is a hitherto unrecognized clotting factor necessary for thromboplastin generation. The mechanism by which the biologic function of this protein is comprised by this group of elements, structurally similar atomically, appears to be reversible chelation at specific sites on the protein essential for its clotting activity. (auth)

• Research Organization: Beth Israel Hospital, Boston and Harvard Univ., Boston
• Sponsoring Organization: USDOE
• NSA Number: NSA-18-019535
• OSTI ID: 4025772
• Journal Information: Journal of Clinical Investigation (U.S.), Vol. Vol: 43; Other Information: Orig. Receipt Date: 31-DEC-64
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
ACTINIDES
ADSORPTION
AMMONIUM COMPOUNDS
BIOLOGY
BLOOD
BLOOD CELLS
BLOOD PLASMA
BLOOD SERUM
CERIUM
CHELATES
CITRIC ACID
COAGULATION
COLLOIDS
EDTA
ENZYMES
GLOBULINS
LANTHANUM
LANTHANUM COMPOUNDS
NEODYMIUM
OXALATES
PLATELETS
PRECIPITATION
PROTEINS
RARE EARTHS
REACTION KINETICS
SULFATES
THORIUM CHLORIDES
THORIUM OXIDES
THOROTRAST
THROMBOPLASTIN
URANIUM