The distribution of single strand breakage at guanine initiated by 193nm light is different for single and double stranded DNA
- Medical Research Council Radiobiology Unit, Chilton (United Kingdom)
- Rutherford Appleton Lab., Chilton (United Kingdom). Central Laser Facility
The DNA sample was denatured in a boiling water bath for 5 mins, then snap frozen at {minus}55 C. Just before irradiation, the sample was thawed and immediately transferred into the photolysis cuvette. Both the control and the denatured DNA solutions were irradiated (193nm) in a quartz cuvette for 2 seconds using the ArF excimer laser at a pulse repetition rate of 10Hz, and subsequently ethanol precipitated. To determine whether 193 nm photolysis of single stranded DNA results in a similar specificity for strand breakage as for double stranded DNA, a known double stranded DNA sequence was 5{prime} end-labelled on one strand, photolyzed in both the single stranded and double stranded form under aerobic conditions and the product resolved by denaturing sequencing gel electrophoresis. Non-random cleavage of the DNA was observed for both samples, as depicted in the autoradiograph. As previously reported and shown again here, the photoinduced cleavage of double stranded DNA shows a selectivity associated with guanine.
- OSTI ID:
- 400728
- Report Number(s):
- CONF-9410280-; ISBN 0-935470-90-5; TRN: IM9650%%134
- Resource Relation:
- Conference: International workshop on radiation damage in DNA: relationships at early times, Gleneden, OR (United States), 1-6 Oct 1994; Other Information: PBD: 1995; Related Information: Is Part Of Radiation damage in DNA: Structure/function relationships at early times; Fuciarelli, A.F. [ed.] [Pacific Northwest Lab., Richland, WA (United States). Biology and Chemistry Dept.]; Zimbrick, J.D. [ed.] [National Academy of Sciences, Washington, DC (United States). National Research Council]; PB: 460 p.
- Country of Publication:
- United States
- Language:
- English
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