Properties of CENP-B and its target sequence in a satellite DNA
- Nagoya Univ. (Japan)
The centromere plays an essential role in the proper segregation of eukaryotic chromosomes at mitosis and meiosis. The centromere is the multifunctional domain of chromosome responsible for sister chromatid association at the inner site and for microtubule attachment at the outer surface. It also acts as a mechanochemical motor for chromosome movement. These multiple centromere functions must, in some way, be directed by a cis-acting DNA sequence located in the centromere region. Indeed, specific centromere DNA sequences (CEN-DNA) were identified in two yeast species. In Saccharomyces cerevisiae, CEN-DNA consists of roughly 125 bp sequence composed of three conserved elements. In contrast, the centromere sequence of S. pombe is quite different from S. cerevisiae in length and sequence organization. The molecular bases for understanding the structure and function of the centromere/kinetochore domain have not been elucidated in higher eukaryotes. In mammalian cells, satellite DNA`s are localized in the centromeric heterochromatin or heterochromatic arm. In all human chromosomes, the alpha satellite or alphoid DNA family, a highly repetitive DNA composed of about 170 bp fundamental monomer repeating units, is found at the primary constriction. Its function, however, has not been established.
- OSTI ID:
- 28429
- Report Number(s):
- CONF-9210475-Cond.; TRN: 95:001845-0003
- Resource Relation:
- Conference: NATO advanced research workshop, Crete (Greece), 10-15 Oct 1992; Other Information: PBD: 1993; Related Information: Is Part Of Chromosome segregation and aneuploidy; Vig, B.K. [ed.] [University of Nevada, Reno, NV (United States). Dept. of Biology]; PB: 429 p.
- Country of Publication:
- United States
- Language:
- English
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