Multigene deletions in lung adenocarcinomas from irradiated and control mice
- Argonne National Lab., IL (United States). Center for Mechanistic Biology and Biotechnology
K-ras codon 12 point mutations mRb and p53 gene deletions were examined in tissues from 120 normal lungs and lung adenocarcinomas that were Formalin-treated and paraffin-embedded 25 years ago. The results showed that 12 of 60 (20%) lung adenocarcinomas had mRb deletions. All lung adenocarcinomas that were initially found bearing deleted mRb had p53 deletions (15 of 15; 100%). A significantly higher mutation frequency for K-ras codon 12 point mutations was also found in the lung adenocarcinomas from mice exposed to 24 once-weekly neutron irradiation (10 of 10; 100%) compared with those exposed to 24 or 60 once-weekly {gamma}-ray doses (5 of 10; 50%). The data suggested that p53 and K-ras gene alterations were two contributory factors responsible for the increased incidence of lung adenocarcinoma in B6CF{sub 1} male mice exposed to protracted neutron radiation.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); National Insts. of Health, Bethesda, MD (United States)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 244586
- Report Number(s):
- ANL/CMB/CP-90034; CONF-9604136-18; ON: DE96011121; CNN: Grant ES07141-02; TRN: 96:014605
- Resource Relation:
- Conference: 2. Argonne National Laboratory technical women`s symposium, Argonne, IL (United States), 29-30 Apr 1996; Other Information: PBD: [1996]
- Country of Publication:
- United States
- Language:
- English
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