LPS-Induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice

Mendez, Melanie E.; Sebastian, Aimy; Murugesh, Deepa K.; Hum, Nicholas R.; McCool, Jillian L.; Hsia, Allison W.; Christiansen, Blaine A.; Loots, Gabriela G.

doi:10.1002/jbmr.4117

Title: LPS-Induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice

Journal Article · Sun Jun 21 00:00:00 EDT 2020 · Journal of Bone and Mineral Research

DOI:https://doi.org/10.1002/jbmr.4117· OSTI ID:2323594

Mendez, Melanie E.; Sebastian, Aimy; Murugesh, Deepa K.; Hum, Nicholas R.; McCool, Jillian L.; Hsia, Allison W.;

;

Osteoarthritis (OA) is a debilitating and painful disease characterized by the progressive loss of articular cartilage. Post-traumatic osteoarthritis (PTOA) is an injury-induced type of OA that persists in an asymptomatic phase for years before it becomes diagnosed in ~50% of injured individuals. Although PTOA is not classified as an inflammatory disease, it has been suggested that inflammation could be a major driver of PTOA development. Here we examined whether a state of systemic inflammation induced by lipopolysaccharide (LPS) administration 5-days before injury would modulate PTOA outcomes. RNA-seq analysis at 1-day post-injury followed by micro-computed tomography (μCT) and histology characterization at 6 weeks post-injury revealed that LPS administration causes more severe PTOA phenotypes. These phenotypes included significantly higher loss of cartilage and subchondral bone volume. Gene expression analysis showed that LPS alone induced a large cohort of inflammatory genes previously shown to be elevated in synovial M1 macrophages of rheumatoid arthritis (RA) patients, suggesting that systemic LPS produces synovitis. This synovitis was sufficient to promote PTOA in MRL/MpJ mice, a strain previously shown to be resistant to PTOA. The synovium of LPS-treated injured joints displayed an increase in cellularity, and immunohistological examination confirmed that this increase was in part attributable to an elevation in type 1 macrophages. LPS induced the expression of Tlr7 and Tlr8 in both injured and uninjured joints, genes known to be elevated in RA. We conclude that inflammation before injury is an important risk factor for the development of PTOA and that correlating patient serum endotoxin levels or their state of systemic inflammation with PTOA progression may help develop new, effective treatments to lower the rate of PTOA in injured individuals.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program; USDOD; National Institutes of Health (NIH); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344; PR180268P1; R01 AR075013

OSTI ID:: 2323594

Alternate ID(s):: OSTI ID: 1815561; OSTI ID: 1867532

Report Number(s):: LLNL-JRNL-797798

Journal Information:: Journal of Bone and Mineral Research, Journal Name: Journal of Bone and Mineral Research Vol. 35 Journal Issue: 11; ISSN 0884-0431

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (69)

The role of cytokines in inflammatory bone loss Souza, Pedro P. C.; Lerner, Ulf H. Immunological Investigations, Vol. 42, Issue 7 https://doi.org/10.3109/08820139.2013.822766	journal	September 2013
Blnk Fu, Chong; Turck, Christoph W.; Kurosaki, Tomohiro Immunity, Vol. 9, Issue 1 https://doi.org/10.1016/S1074-7613(00)80591-9	journal	July 1998
Commensal bacteria at the interface of host metabolism and the immune system Brestoff, Jonathan R.; Artis, David Nature Immunology, Vol. 14, Issue 7 https://doi.org/10.1038/ni.2640	journal	June 2013
Fate of the ACL-injured Patient: A Prospective Outcome Study Daniel, Dale M.; Stone, Mary Lou; Dobson, Barbara E. The American Journal of Sports Medicine, Vol. 22, Issue 5 https://doi.org/10.1177/036354659402200511	journal	September 1994
Plasma Endotoxin Concentrations in Patients with Urinary Tract Infections Goto, Toshihiro; Makinose, Shinichi; Ohi, Yoshitada International Journal of Urology, Vol. 2, Issue 4 https://doi.org/10.1111/j.1442-2042.1995.tb00464.x	journal	September 1995
Non-invasive mouse models of post-traumatic osteoarthritis Christiansen, B. A.; Guilak, F.; Lockwood, K. A. Osteoarthritis and Cartilage, Vol. 23, Issue 10 https://doi.org/10.1016/j.joca.2015.05.009	journal	October 2015
Notum deacylates Wnt proteins to suppress signalling activity Kakugawa, Satoshi; Langton, Paul F.; Zebisch, Matthias Nature, Vol. 519, Issue 7542 https://doi.org/10.1038/nature14259	journal	February 2015
Intermediate monocytes are increased in enthesitis-related arthritis, a category of juvenile idiopathic arthritis Gaur, P.; Myles, A.; Misra, R. Clinical and Experimental Immunology, Vol. 187, Issue 2 https://doi.org/10.1111/cei.12880	journal	October 2016
Comparative Transcriptomics Identifies Novel Genes and Pathways Involved in Post-Traumatic Osteoarthritis Development and Progression Sebastian, Aimy; Chang, Jiun; Mendez, Melanie International Journal of Molecular Sciences, Vol. 19, Issue 9 https://doi.org/10.3390/ijms19092657	journal	September 2018
Bone Response to Loading in Mice With Targeted Disruption of the Cartilage Oligomeric Matrix Protein Gene Sengul, A.; Mohan, S.; Kesavan, C. Physiological Research https://doi.org/10.33549/physiolres.932307	journal	December 2012
Immunohistochemical analysis of an equine model of synovitis-induced arthritis Todhunter, P. G.; Kincaid, S. A.; Todhunter, R. J. American Journal of Veterinary Research, Vol. 57, Issue 7 https://doi.org/10.2460/ajvr.1996.57.07.1080	journal	July 1996
Osteoarthritis induced by destabilization of the medial meniscus is reduced in germ-free mice Ulici, V.; Kelley, K. L.; Azcarate-Peril, M. A. Osteoarthritis and Cartilage, Vol. 26, Issue 8 https://doi.org/10.1016/j.joca.2018.05.016	journal	August 2018
Expression of the B-Cell Receptor Component CD79a on Immature Myeloid Cells Contributes to Their Tumor Promoting Effects Luger, Dror; Yang, Yu-an; Raviv, Asaf PLoS ONE, Vol. 8, Issue 10 https://doi.org/10.1371/journal.pone.0076115	journal	October 2013
Inflammation in joint injury and post-traumatic osteoarthritis Lieberthal, J.; Sambamurthy, N.; Scanzello, C. R. Osteoarthritis and Cartilage, Vol. 23, Issue 11 https://doi.org/10.1016/j.joca.2015.08.015	journal	November 2015
Pericellular Matrilins Regulate Activation of Chondrocytes by Cyclic Load-Induced Matrix Deformation Kanbe, Katsuaki; Yang, Xu; Wei, Lei Journal of Bone and Mineral Research, Vol. 22, Issue 2 https://doi.org/10.1359/jbmr.061104	journal	November 2006
Acute and late changes in intraarticular cytokine levels following anterior cruciate ligament injury: CYTOKINE LEVELS FOLLOWING ACL INJURY Bigoni, Marco; Sacerdote, Paola; Turati, Marco Journal of Orthopaedic Research, Vol. 31, Issue 2 https://doi.org/10.1002/jor.22208	journal	August 2012
Effects of intraarticular ropivacaine and morphine on lipopolysaccharide‐induced synovitis in horses Santos, Luiz César Pereira; De Moraes, Aury Nunes; Saito, Mere Erika Veterinary Anaesthesia and Analgesia, Vol. 36, Issue 3 https://doi.org/10.1111/j.1467-2995.2009.00452.x	journal	May 2009
The Chemokine CCL6 Promotes Innate Immunity via Immune Cell Activation and Recruitment Coelho, Ana L.; Schaller, Matthew A.; Benjamim, Claudia F. The Journal of Immunology, Vol. 179, Issue 8 https://doi.org/10.4049/jimmunol.179.8.5474	journal	October 2007
SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury: SOST OVEREXPRESSION IMPROVES PTOA OUTCOMES Chang, Jiun C.; Christiansen, Blaine A.; Murugesh, Deepa K. Journal of Bone and Mineral Research, Vol. 33, Issue 6 https://doi.org/10.1002/jbmr.3397	journal	April 2018
Single-nucleotide polymorphism in the hyaluronan and proteoglycan link protein 1 (HAPLN1) gene is associated with spinal osteophyte formation and disc degeneration in Japanese women Urano, Tomohiko; Narusawa, Ken’ichiro; Shiraki, Masataka European Spine Journal, Vol. 20, Issue 4 https://doi.org/10.1007/s00586-010-1598-0	journal	October 2010
Conditional Deletion of Indian Hedgehog in Limb Mesenchyme Results in Complete Loss of Growth Plate Formation but Allows Mature Osteoblast Differentiation: CONDITIONAL DELETION OF IHH IN MOUSE LIMB MESENCHYME Amano, Katsuhiko; Densmore, Michael J.; Lanske, Beate Journal of Bone and Mineral Research, Vol. 30, Issue 12 https://doi.org/10.1002/jbmr.2582	journal	July 2015
Post-traumatic osteoarthritis: Improved understanding and opportunities for early intervention Anderson, Donald D.; Chubinskaya, Susan; Guilak, Farshid Journal of Orthopaedic Research, Vol. 29, Issue 6 https://doi.org/10.1002/jor.21359	journal	February 2011
Inflammatory bone loss: pathogenesis and therapeutic intervention Redlich, Kurt; Smolen, Josef S. Nature Reviews Drug Discovery, Vol. 11, Issue 3 https://doi.org/10.1038/nrd3669	journal	March 2012
Comparison of loading rate-dependent injury modes in a murine model of post-traumatic osteoarthritis: COMPARISON OF PTOA INJURY MODES Lockwood, Kevin A.; Chu, Bryce T.; Anderson, Matthew J. Journal of Orthopaedic Research, Vol. 32, Issue 1 https://doi.org/10.1002/jor.22480	journal	September 2013
TLR5, a Novel and Unidentified Inflammatory Mediator in Rheumatoid Arthritis that Correlates with Disease Activity Score and Joint TNF-α Levels Chamberlain, Nathan D.; Vila, Olga M.; Volin, Michael V. The Journal of Immunology, Vol. 189, Issue 1 https://doi.org/10.4049/jimmunol.1102977	journal	June 2012
Osteoimmunology: Interactions of the Bone and Immune System Lorenzo, Joseph; Horowitz, Mark; Choi, Yongwon Endocrine Reviews, Vol. 29, Issue 4 https://doi.org/10.1210/er.2007-0038	journal	May 2008
Ligation of TLR5 Promotes Myeloid Cell Infiltration and Differentiation into Mature Osteoclasts in Rheumatoid Arthritis and Experimental Arthritis Kim, Seung-jae; Chen, Zhenlong; Chamberlain, Nathan D. The Journal of Immunology, Vol. 193, Issue 8 https://doi.org/10.4049/jimmunol.1302998	journal	September 2014
Epigenetic Programing of B-Cell Lymphoma by BCL6 and Its Genetic Deregulation Yang, Haopeng; Green, Michael R. Frontiers in Cell and Developmental Biology, Vol. 7 https://doi.org/10.3389/fcell.2019.00272	journal	November 2019
Common Polymorphisms in the Prostaglandin Pathway Genes and Their Association with Breast Cancer Susceptibility and Survival Abraham, Jean E.; Harrington, Patricia; Driver, Kristy E. Clinical Cancer Research, Vol. 15, Issue 6 https://doi.org/10.1158/1078-0432.CCR-08-0716	journal	March 2009
Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice Deng, Zhenhan; Gao, Xueqin; Sun, Xuying The FASEB Journal, Vol. 33, Issue 8 https://doi.org/10.1096/fj.201802132RR	journal	May 2019
Guidelines for assessment of bone microstructure in rodents using micro-computed tomography Bouxsein, Mary L.; Boyd, Stephen K.; Christiansen, Blaine A. Journal of Bone and Mineral Research, Vol. 25, Issue 7 https://doi.org/10.1002/jbmr.141	journal	June 2010
Inflammation in bone physiology and pathology Adamopoulos, Iannis E. Current Opinion in Rheumatology, Vol. 30, Issue 1 https://doi.org/10.1097/BOR.0000000000000449	journal	January 2018
Function, osteoarthritis and activity after ACL-rupture: 11 years follow-up results of conservative versus reconstructive treatment Kessler, M. A.; Behrend, H.; Henz, S. Knee Surgery, Sports Traumatology, Arthroscopy, Vol. 16, Issue 5 https://doi.org/10.1007/s00167-008-0498-x	journal	February 2008
The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the mouse Glasson, S. S.; Chambers, M. G.; Van Den Berg, W. B. Osteoarthritis and Cartilage, Vol. 18 https://doi.org/10.1016/j.joca.2010.05.025	journal	October 2010
Cloning of a T Cell Growth Factor that Interacts with the β Chain of the Interleukin-2 Receptor Grabstein, Kenneth H.; Eisenman, June; Shanebeck, Kurt Science, Vol. 264, Issue 5161 https://doi.org/10.1126/science.8178155	journal	May 1994
Identification and characterization of the novel Col10a1 regulatory mechanism during chondrocyte hypertrophic differentiation Gu, J.; Lu, Y.; Li, F. Cell Death & Disease, Vol. 5, Issue 10 https://doi.org/10.1038/cddis.2014.444	journal	October 2014
Nanomechanical phenotype of chondroadherin-null murine articular cartilage Batista, Michael A.; Nia, Hadi T.; Önnerfjord, Patrik Matrix Biology, Vol. 38 https://doi.org/10.1016/j.matbio.2014.05.008	journal	September 2014
PKR regulates LPS-induced osteoclast formation and bone destruction in vitro and in vivo Teramachi, J.; Inagaki, Y.; Shinohara, H. Oral Diseases, Vol. 23, Issue 2 https://doi.org/10.1111/odi.12592	journal	November 2016
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes Chamberlain, Nathan D.; Kim, Seung-jae; Vila, Olga M. Annals of the Rheumatic Diseases, Vol. 72, Issue 3 https://doi.org/10.1136/annrheumdis-2011-201203	journal	June 2012
Role of CC chemokine CCL6/C10 as a monocyte chemoattractant in a murine acute peritonitis LaFleur, Andrew M.; Lukacs, Nicholas W.; Kunkel, Steven L. Mediators of Inflammation, Vol. 13, Issue 5-6 https://doi.org/10.1080/09629350400014172	journal	January 2004
Induction of human mammary-associated serum amyloid A3 expression by prolactin or lipopolysaccharide Larson, Marilynn A.; Wei, Shu H.; Weber, Annika Biochemical and Biophysical Research Communications, Vol. 301, Issue 4 https://doi.org/10.1016/S0006-291X(03)00045-7	journal	February 2003
Association of protein kinase C alpha (PRKCA) gene with multiple sclerosis in a UK population Barton, A. Brain, Vol. 127, Issue 8 https://doi.org/10.1093/brain/awh193	journal	June 2004
CCR2 ⁺ Inflammatory Monocytes Are Recruited to Yersinia pseudotuberculosis Pyogranulomas and Dictate Adaptive Responses at the Expense of Innate Immunity during Oral Infection Zhang, Yue; Khairallah, Camille; Sheridan, Brian S. Infection and Immunity, Vol. 86, Issue 3 https://doi.org/10.1128/IAI.00782-17	journal	March 2018
Isoflurane Prys-Roberts, C. British Journal of Anaesthesia, Vol. 53, Issue 12 https://doi.org/10.1093/bja/53.12.1243	journal	December 1981
CD22: A Regulator of Innate and Adaptive B Cell Responses and Autoimmunity Clark, Edward A.; Giltiay, Natalia V. Frontiers in Immunology, Vol. 9 https://doi.org/10.3389/fimmu.2018.02235	journal	September 2018
CXCL1/CXCR2 signaling in pathological pain: Role in peripheral and central sensitization Silva, Rangel L.; Lopes, Alexandre H.; Guimarães, Rafaela M. Neurobiology of Disease, Vol. 105 https://doi.org/10.1016/j.nbd.2017.06.001	journal	September 2017
Locally renewing resident synovial macrophages provide a protective barrier for the joint Culemann, Stephan; Grüneboom, Anika; Nicolás-Ávila, José Ángel Nature, Vol. 572, Issue 7771 https://doi.org/10.1038/s41586-019-1471-1	journal	August 2019
Serum Amyloid A (apoSAA) Expression Is Up-Regulated in Rheumatoid Arthritis and Induces Transcription of Matrix Metalloproteinases Vallon, Rüdiger; Freuler, Felix; Desta-Tsedu, Netsanet The Journal of Immunology, Vol. 166, Issue 4 https://doi.org/10.4049/jimmunol.166.4.2801	journal	February 2001
Absence of posttraumatic arthritis following intraarticular fracture in the MRL/MpJ mouse Ward, Benjamin D.; Furman, Bridgette D.; Huebner, Janet L. Arthritis & Rheumatism, Vol. 58, Issue 3 https://doi.org/10.1002/art.23288	journal	January 2008
Musculoskeletal changes following non-invasive knee injury using a novel mouse model of post-traumatic osteoarthritis Christiansen, B. A.; Anderson, M. J.; Lee, C. A. Osteoarthritis and Cartilage, Vol. 20, Issue 7 https://doi.org/10.1016/j.joca.2012.04.014	journal	July 2012
The B-Cell-Specific src-Family Kinase Blk Is Dispensable for B-Cell Development and Activation Texido, Gemma; Su, I-hsin; Mecklenbräuker, Ingrid Molecular and Cellular Biology, Vol. 20, Issue 4 https://doi.org/10.1128/MCB.20.4.1227-1233.2000	journal	February 2000
Sclerostin antibody treatment improves fracture outcomes in a Type I diabetic mouse model Yee, Cristal S.; Xie, LiQin; Hatsell, Sarah Bone, Vol. 82 https://doi.org/10.1016/j.bone.2015.04.048	journal	January 2016
Cartilage glycosaminoglycan loss in the acute phase after an anterior cruciate ligament injury: Delayed gadolinium-enhanced magnetic resonance imaging of cartilage and synovial fluid analysis Tiderius, Carl Johan; Olsson, Lars Eric; Nyquist, Fredrik Arthritis & Rheumatism, Vol. 52, Issue 1 https://doi.org/10.1002/art.20795	journal	January 2005
Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases Viegas, Carla S. B.; Costa, Rúben M.; Santos, Lúcia PLOS ONE, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pone.0177829	journal	May 2017
Involvement of gut microbiome in human health and disease: brief overview, knowledge gaps and research opportunities Liang, Dachao; Leung, Ross Ka-Kit; Guan, Wenda Gut Pathogens, Vol. 10, Issue 1 https://doi.org/10.1186/s13099-018-0230-4	journal	January 2018
CD4 ⁺ T Cell Expressed CD80 Regulates Central Nervous System Effector Function and Survival during Experimental Autoimmune Encephalomyelitis Podojil, Joseph R.; Kohm, Adam P.; Miller, Stephen D. The Journal of Immunology, Vol. 177, Issue 5 https://doi.org/10.4049/jimmunol.177.5.2948	journal	August 2006
Notch signaling inhibition protects against LPS mediated osteolysis Goel, Peeyush N.; Egol, Alexander J.; Moharrer, Yasaman Biochemical and Biophysical Research Communications, Vol. 515, Issue 4 https://doi.org/10.1016/j.bbrc.2019.05.166	journal	August 2019
Bone loss in inflammatory disorders Hardy, R.; Cooper, M. S. Journal of Endocrinology, Vol. 201, Issue 3 https://doi.org/10.1677/JOE-08-0568	journal	February 2009
Tissue-specific Role of CX ₃ CR1 Expressing Immune Cells and Their Relationships with Human Disease Lee, Myoungsoo; Lee, Yongsung; Song, Jihye Immune Network, Vol. 18, Issue 1 https://doi.org/10.4110/in.2018.18.e5	journal	January 2018
RNA recognition by human TLR8 can lead to autoimmune inflammation Guiducci, Cristiana; Gong, Mei; Cepika, Alma-Martina Journal of Experimental Medicine, Vol. 210, Issue 13 https://doi.org/10.1084/jem.20131044	journal	November 2013
Sexual dimorphisms in leukocyte trafficking in a mouse peritonitis model Kay, E.; Gomez-Garcia, L.; Woodfin, A. Journal of Leukocyte Biology, Vol. 98, Issue 5 https://doi.org/10.1189/jlb.3A1214-601RR	journal	July 2015
Brain Region-dependent Heterogeneity and Dose-dependent Difference in Transient Microglia Population Increase during Lipopolysaccharide-induced Inflammation Furube, Eriko; Kawai, Shintaro; Inagaki, Haruna Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-20643-3	journal	February 2018
Collagen IX-deficiency seriously compromises growth cartilage development in mice Dreier, R.; Opolka, A.; Grifka, J. Matrix Biology, Vol. 27, Issue 4 https://doi.org/10.1016/j.matbio.2008.01.006	journal	May 2008
Experimentally-induced synovitis as a model for acute synovitis in the horse Palmer, Jan L.; Bertone, Alicia L. Equine Veterinary Journal, Vol. 26, Issue 6 https://doi.org/10.1111/j.2042-3306.1994.tb04056.x	journal	November 1994
Cytokine-like 1 Knock-out Mice ( Cytl1 −/− ) Show Normal Cartilage and Bone Development but Exhibit Augmented Osteoarthritic Cartilage Destruction Jeon, Jimin; Oh, Hwanhee; Lee, Gyusuk Journal of Biological Chemistry, Vol. 286, Issue 31 https://doi.org/10.1074/jbc.M111.218065	journal	June 2011
Effects of intra-articularly administered endotoxin on clinical signs of disease and synovial fluid tumor necrosis factor, interleukin 6, and prostaglandin E2 values in horses Hawkins, Dan L.; MacKay, Robert J.; Gum, Glen G. American Journal of Veterinary Research, Vol. 54, Issue 3 https://doi.org/10.2460/ajvr.1993.54.03.379	journal	March 1993
Global molecular changes in a tibial compression induced ACL rupture model of post-traumatic osteoarthritis: GLOBAL MOLECULAR CHANGES AFTER ACL INJURY Chang, Jiun C.; Sebastian, Aimy; Murugesh, Deepa K. Journal of Orthopaedic Research, Vol. 35, Issue 3 https://doi.org/10.1002/jor.23263	journal	May 2016
Review of current understanding of post-traumatic osteoarthritis resulting from sports injuries: REVIEW OF UNDERSTANDING OF PTOA FROM SPORTS INJURIES Carbone, Andrew; Rodeo, Scott Journal of Orthopaedic Research, Vol. 35, Issue 3 https://doi.org/10.1002/jor.23341	journal	July 2016
Usefulness of CD79b Expression in the Diagnosis of B-Cell Chronic Lymphoproliferative Disorders McCarron, Karen F.; Hammel, Jeffrey P.; Hsi, Eric D. American Journal of Clinical Pathology, Vol. 113, Issue 6 https://doi.org/10.1309/G689-2J36-KCTC-WD5N	journal	June 2000

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Title: LPS-Induced Inflammation Prior to Injury Exacerbates the Development of Post-Traumatic Osteoarthritis in Mice

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