Drosophila intestinal homeostasis requires CTP synthase

Journal Article · Mon Nov 15 00:00:00 EST 2021 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2021.112838· OSTI ID:23195468

Zhou, Youfang ^[1]; Liu, Jingnan ^[1]; Zhang, Yuanbing ^[1]; Liu, Ji-Long ^[1]

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1. School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210 (China)

CTP synthase (CTPS) senses all four nucleotides and forms filamentous structures termed cytoophidia in all three domains of life. How CTPS and cytoophidia function in a developmental context, however, remains underexplored. We report that CTPS forms cytoophidia in a subset of cells in the Drosophila midgut. We found that cytoophidia exist in intestinal stem cells (ISC) and enteroblasts in similar proportions. Both refeeding after starvation and feeding with dextran sulfate sodium (DSS) induce ISC proliferation and elongate cytoophidia. Knockdown of CTPS inhibits ISC proliferation. Remarkably, disruption of CTPS cytoophidia inhibits DSS-induced ISC proliferation. Taken together, these data suggest that both the expression level and the filament-form property of CTPS are crucial for intestinal homeostasis in Drosophila.

• OSTI ID: 23195468
• Journal Information: Experimental Cell Research, Vol. 408, Issue 1; Other Information: Copyright (c) 2021 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DEXTRAN
DROSOPHILA
FASTING
FEEDING
FILAMENTS
HOMEOSTASIS
NUCLEOTIDES
SODIUM
STEM CELLS
SULFATES