Binding of SRSF4 to a novel enhancer modulates splicing of exon 6 of Fas pre-mRNA
- School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 500-712, South (Korea, Republic of)
Highlights: • SRSF4 expression promotes exon 6 inclusion of Fas pre-mRNA. • A stronger 5′ splice-site of exon 6 is required for SRSF4-mediated regulation on exon 6 splicing. • SRSF4 functionally targets GGGGUG RNA sequence on exon 6. • SRSF4 directly interacts with GGGGUG sequence on exon 6. Alternative splicing of exon 6 in Fas pre-mRNA generates a membrane bound pro-apoptotic isoform or soluble anti-apoptotic isoform. SRSF4 is a member of Arginine-Serine rich (SR) protein family. Here we demonstrate that increased SRSF4 expression stimulates exon 6 inclusion, and that reduced SRSF4 expression promotes exon 6 exclusion. We also show that weaker but not stronger 5’ splice-site strength of exon 6 abolishes the SRSF4 effects on exon 6 splicing. Furthermore, we identified a novel enhancer on exon 6, on which SRSF4 interacts functionally and physically. Our results illustrate a novel regulatory mechanism of Fas pre-mRNA splicing.
- OSTI ID:
- 23137243
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 506, Issue 3; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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