Delavatine A, an unusual isoquinoline alkaloid exerts anti-inflammation on LPS-induced proinflammatory cytokines production by suppressing NF-κB activation in BV-2 microglia
- School of Pharmacy, Jiangsu University, Zhenjiang, 212013 (China)
- Department of Phytochemistry, School of Pharmacy, The Second Military Medical University, Shanghai, 200433 (China)
Highlights: • The anti-inflammation of delavatine A was first discovered. • The activity was proved not only by the production of NO, TNF-α, IL-6 and IL-1β, but the regulatory genes including iNOS, COX-2, TNF-a, IL-6 and IL-1β. • Delavatine A regulated the LPS-induced proinflammatory cytokines production by suppressing NF-κB activation in BV-2 microglia. Delavatine A, an unusual isoquinoline alkaloid isolated from I. delavayi, was first studied for anti-inflammatory effect using lipopolysaccharide (LPS)-induced BV-2 microglia. In the present study, we found that delavatine A substantially suppressed the LPS-induced pro-inflammatory mediators, nitric oxide (NO), and tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1β (IL-1β) in BV-2 microglial cells. These effects resulted from the inhibition of their regulatory genes inducible NO synthase (iNOS), cycloxygenase-2 (COX-2) and TNF-a, IL-6, IL-1β. In addition, we examined several pathways related to inflammation. The results revealed that delavatine A significantly decreased LPS-induced the activation of nuclear factor-κB (NF-κB) by suppressing the p65 subunits, and the phosphorylation of IκBα, while not related to PI3K/Akt or MAPK pathways.
- OSTI ID:
- 23105746
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 502, Issue 2; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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