Long noncoding RNA LINC00858 promotes osteosarcoma through regulating miR-139-CDK14 axis
- Department of Orthopedic, The 117 Hospital of The PLA, Hangzhou, Zhejiang, 310012 (China)
Highlights: • LncRNA LINC00858 was significantly upregulated in both osteosarcoma tissues and cell lines. • LINC00858 silencing repressed osteosarcoma cells’ proliferation and invasion, and inhibited the tumor growth. • LINC00858 was identified to sponge miR-139 to form RISC using luciferase reporter and RIP. • LINC00858/miR-139/CDK14 axis promotes the tumorigenesis of osteosarcoma. Long noncoding RNAs (lncRNAs) have been identified to modulate the tumorigenesis of human cancers. The in-depth of lncRNAs on human osteosarcoma oncogenesis is still ambiguous. In present study, functional and mechanism experiments were conducted to investigate the role of long intergenic non-protein coding RNA 00858 (LINC00858) on human osteosarcoma tumorigenesis. Results demonstrated that LINC00858 expression was significantly upregulated in both osteosarcoma tissues and cell lines. Mechanism assays presented that LINC00858 silencing significantly repressed osteosarcoma cells' proliferation and invasion in vitro, and inhibited the tumor growth in vivo. In further experiments, LINC00858 was identified to sponge miR-139 to form RNA-induced silencing complex (RISC) using luciferase reporter assay and RNA immunoprecipitation (RIP). Besides, CDK14 was validated to be the target protein the miR-139. Rescue experiments confirmed the role of LINC00858/miR-139/CDK14 pathway on osteosarcoma cells' phenotype. In summary, these data prove that LINC00858/miR-139/CDK14 axis promotes the tumorigenesis of osteosarcoma, providing a new mechanism or target for osteosarcoma.
- OSTI ID:
- 23105630
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 503, Issue 2; Other Information: Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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