NAMPT regulates senescence, proliferation, and migration of endothelial progenitor cells through the SIRT1 AS lncRNA/miR-22/SIRT1 pathway
- Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, Hunan (China)
- Department of Physiatry, Xiangya Hospital, Central South University, Changsha 410008, Hunan (China)
- Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, Hunan (China)
- Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, Hunan (China)
The importance of endothelial progenitor cells (EPCs) in cardiovascular diseases has been demonstrated by numerous studies. Previous studies have shown that Nicotinamide phosphoribosyltransferase (NAMPT) plays a role in EPC development by regulating Sirtuin 1 (SIRT1), but the specific mechanism has not yet been elucidated. After stimulating EPCs with NAMPT, expression of SIRT1 and SIRT1 antisense long non-coding RNA (AS lncRNA) was upregulated. Upon transfection of an SIRT1 AS lncRNA overexpression vector into EPCs, SIRT1 expression was upregulated. Upon transfection of a small interfering RNA (siRNA) that targets SIRT1 AS lncRNA along with NAMPT, SIRT1 AS lncRNA was downregulated and NAMPT-induced SIRT1 expression was reduced. We used software analyses and a dual-luciferase reporter assay to demonstrate that microRNA (miR)-22 regulated SIRT1 and SIRT1 AS lncRNA. Our data suggest that SIRT1 AS lncRNA relieves miR-22-induced SIRT1 downregulation by competitively sponging miR-22. By measuring EPC senescence, proliferation, and migration, we found that NAMPT inhibited EPC senescence through an SIRT1 AS lncRNA/miR-22/SIRT1 pathway and promoted EPC proliferation and migration. These findings provide a new theoretical basis for the prevention and treatment of atherosclerosis (AS) and other cardiovascular diseases. - Highlights: • NAMPT upregulates expression of SIRT1 AS lncRNA, which regulates SIRT1 expression in EPCs. • NAMPT regulates SIRT1 expression through an SIRT1 AS lncRNA/miR-22 pathway. • NAMPT regulates EPC senescence through an SIRT1 AS lncRNA/miR-22/SIRT1 pathway. • NAMPT regulates EPC proliferation and migration through an SIRT1 AS lncRNA/miR-22/SIRT1 pathway.
- OSTI ID:
- 22696616
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 478, Issue 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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