Functional analysis of Salix purpurea genes support roles for ARR17 and GATA15 as master regulators of sex determination
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- Horticulture Section, School of Integrative Plant Science Cornell University, Cornell AgriTech Geneva New York USA, Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee USA
- Biosciences Division Oak Ridge National Laboratory Oak Ridge Tennessee USA
- Lawrence Berkeley National Laboratory US Department of Energy Joint Genome Institute Berkeley California USA
- Horticulture Section, School of Integrative Plant Science Cornell University, Cornell AgriTech Geneva New York USA
Abstract The Salicaceae family is of growing interest in the study of dioecy in plants because the sex determination region (SDR) has been shown to be highly dynamic, with differing locations and heterogametic systems between species. Without the ability to transform and regenerate Salix in tissue culture, previous studies investigating the mechanisms regulating sex in the genus Salix have been limited to genome resequencing and differential gene expression, which are mostly descriptive in nature, and functional validation of candidate sex determination genes has not yet been conducted. Here, we used Arabidopsis to functionally characterize a suite of previously identified candidate genes involved in sex determination and sex dimorphism in the bioenergy shrub willow Salix purpurea . Six candidate master regulator genes for sex determination were heterologously expressed in Arabidopsis, followed by floral proteome analysis. In addition, 11 transcription factors with predicted roles in mediating sex dimorphism downstream of the SDR were tested using DAP‐Seq in both male and female S. purpurea DNA. The results of this study provide further evidence to support models for the roles of ARR17 and GATA15 as master regulator genes of sex determination in S. purpurea , contributing to a regulatory system that is notably different from that of its sister genus Populus . Evidence was also obtained for the roles of two transcription factors, an AP2 / ERF family gene and a homeodomain‐like transcription factor, in downstream regulation of sex dimorphism.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Office of Workforce Development for Teachers & Scientists (WDTS)
- Grant/Contract Number:
- DE‐SC0014664; DE‐AC02‐05CH11231; DE‐AC05‐00OR22725; AC05-00OR22725; SC0014664; AC02-05CH11231
- OSTI ID:
- 2212828
- Alternate ID(s):
- OSTI ID: 2217716; OSTI ID: 2228391
- Journal Information:
- Plant Direct, Journal Name: Plant Direct Vol. 7 Journal Issue: 11; ISSN 2475-4455
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
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