Reply to Popescu and Zionjic
Journal Article
·
· American Journal of Human Genetics
OSTI ID:219872
- Center for Cellular and Molecular Biology, Hydrabad (India)
- Specialty Diagnosis, La Mirada, CA (United States)
- Southwestern Medical School, Dallas, TX (United States)
- UCLA School of Medicine/VA Medical Center, West Los Angeles, CA (United States)
In our studies, we analyzed eight different cervical cell lines, including two human papilloma virus (HPV)-negative cell lines, for rearrangements on chromosome 11. A number of approaches including RFLP, pulsed-field gel electrophoresis (PFGE), karyotyping, and FISH with 11 painting and 11q13-specific probes were used. Rearrangement of 11q13 sequences, translocation in two (HeLa and CasKi), deletion in one (SiHa), and translocation and deletion in one (C33A) were observed. One other cell line (HT3) showed rearrangement in PFGE gels. Metaphase spreads were not available for FISH studies on this cell line. Thus, 11q13 rearrangement was a nonrandom event. 8 refs.
- OSTI ID:
- 219872
- Journal Information:
- American Journal of Human Genetics, Vol. 58, Issue 2; Other Information: PBD: Feb 1996
- Country of Publication:
- United States
- Language:
- English
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