Progress in Cell Marking for Synchrotron X-ray Computed Tomography

Journal Article · Fri Jul 23 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3478198· OSTI ID:21415286

Hall, Christopher ^[1]; Sturm, Erica ^[2]; Schultke, Elisabeth ^[3]; Arfelli, Fulvia; Astolfo, Alberto ^[4]; Menk, Ralf-Hendrik ^[5]; Juurlink, Bernhard H. J. ^[6]

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1. Monash Centre for Synchrotron Science, Monash University, Imaging and Medical Beam Line, Australian Synchrotron (Australia)
2. Department of Medical Imaging and Radiation Sciences, Monash University (Australia)
3. Dept of Stereotactic Neurosurgery, Albert Ludwigs University of Freiburg (Germany)
4. Department of Physics, University of Trieste, Trieste (Italy)
5. Sincrotrone Trieste SCpA, Trieste (Italy)
6. College of Medicine, Alfaisal University, Riyadh (Saudi Arabia)

Recently there has been an increase in research activity into finding ways of marking cells in live animals for pre-clinical trials. Development of certain drugs and other therapies crucially depend on tracking particular cells or cell types in living systems. Therefore cell marking techniques are required which will enable longitudinal studies, where individuals can be examined several times over the course of a therapy or study. The benefits of being able to study both disease and therapy progression in individuals, rather than cohorts are clear. The need for high contrast 3-D imaging, without harming or altering the biological system requires a non-invasive yet penetrating imaging technique. The technique will also have to provide an appropriate spatial and contrast resolution. X-ray computed tomography offers rapid acquisition of 3-D images and is set to become one of the principal imaging techniques in this area. Work by our group over the last few years has shown that marking cells with gold nano-particles (GNP) is an effective means of visualising marked cells in-vivo using x-ray CT. Here we report the latest results from these studies. Synchrotron X-ray CT images of brain lesions in rats taken using the SYRMEP facility at the Elettra synchrotron in 2009 have been compared with histological examination of the tissues. Some deductions are drawn about the visibility of the gold loaded cells in both light microscopy and x-ray imaging.

• OSTI ID: 21415286
• Journal Information: AIP Conference Proceedings, Vol. 1266, Issue 1; Conference: 6. international conference on medical applications of synchrotron radiation, Melbourne (Australia), 15-18 Feb 2010; Other Information: DOI: 10.1063/1.3478198; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
BRAIN
CAT SCANNING
CLINICAL TRIALS
DISEASES
GOLD
IMAGE PROCESSING
IMAGES
IN VIVO
MICROSCOPY
NUCLEAR MEDICINE
RADIOTHERAPY
RATS
RESOLUTION
SYNCHROTRON RADIATION
THREE-DIMENSIONAL CALCULATIONS
X RADIATION
ANIMALS
BODY
BREMSSTRAHLUNG
CENTRAL NERVOUS SYSTEM
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
ELECTROMAGNETIC RADIATION
ELEMENTS
IONIZING RADIATIONS
MAMMALS
MEDICINE
METALS
NERVOUS SYSTEM
ORGANS
PROCESSING
RADIATIONS
RADIOLOGY
RODENTS
TESTING
THERAPY
TOMOGRAPHY
TRANSITION ELEMENTS
VERTEBRATES