High-fat diet exacerbates inflammation and cell survival signals in the skin of ultraviolet B-irradiated C57BL/6 mice

Journal Article · Tue Dec 15 00:00:00 EST 2009 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2009.09.003· OSTI ID:21344813

Meeran, Syed M; Singh, Tripti ^[1]; Nagy, Tim R ^[2]

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1. Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294 (United States)
2. Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294 (United States)

Inflammation induced by chronic exposure to ultraviolet (UV) radiation has been implicated in various skin diseases. We formulated the hypothesis that a high-fat diet may influence the UV-induced inflammatory responses in the skin. C57BL/6 mice were fed a high-fat diet or control diet and exposed to UVB radiation (120 mJ/cm{sup 2}) three times/week for 10 weeks. The mice were then sacrificed and skin and plasma samples collected for analysis of biomarkers of inflammatory responses using immunohistochemistry, western blotting, ELISA and real-time PCR. We found that the levels of inflammatory biomarkers were increased in the UVB-exposed skin of the mice fed the high-fat diet than the UVB-exposed skin of the mice fed the control diet. The levels of inflammatory biomarkers of early responses to UVB exposure (e.g., myeloperoxidase, cyclooxygenase-2, prostaglandin-E{sub 2}), proinflammatory cytokines (i.e., tumor necrosis factor-alpha, interleukin-1beta, interleukin-6), and proliferating cell nuclear antigen and cell survival signals (phosphatidylinositol-3-kinase and p-Akt-Ser{sup 473}) were higher in high-fat-diet-fed mouse skin than control-diet-fed mouse skin. The plasma levels of insulin growth factor-1 were greater in the UVB-irradiated mice fed the high-fat diet than the UVB-irradiated mice fed the control diet, whereas the levels of plasma adiponectin were significantly lower. This pronounced exacerbation of the UVB-induced inflammatory responses in the skin of mice fed a high-fat diet suggests that high-fat diet may increase susceptibility to inflammation-associated skin diseases, including the risk of skin cancer.

• OSTI ID: 21344813
• Journal Information: Toxicology and Applied Pharmacology, Vol. 241, Issue 3; Other Information: DOI: 10.1016/j.taap.2009.09.003; PII: S0041-008X(09)00374-3; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIGENS
BIOLOGICAL MARKERS
CHRONIC EXPOSURE
DIET
ENZYME IMMUNOASSAY
INFLAMMATION
INSULIN
LYMPHOKINES
MICE
SKIN
SKIN DISEASES
TOXICITY
ULTRAVIOLET RADIATION
ANIMALS
BIOASSAY
BODY
DISEASES
ELECTROMAGNETIC RADIATION
GROWTH FACTORS
HORMONES
IMMUNOASSAY
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HORMONES
PROTEINS
RADIATIONS
RODENTS
SYMPTOMS
VERTEBRATES