ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion
- Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, Av. Para, 1720, Campus Umuarama, Uberlandia, 38400-902, MG (Brazil)
- Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Rua Botucatu, 862, 6o andar, 04023-062 Sao Paulo, SP (Brazil)
- UMR144 CNRS, Institut Curie, 26 rue d'Ulm, F-75248 Paris (France)
Toxoplasma gondii infects a variety of different cell types in a range of different hosts. Host cell invasion by T. gondii occurs by active penetration of the host cell, a process previously described as independent of host actin polymerization. Also, the parasitophorous vacuole has been shown to resist fusion with endocytic and exocytic pathways of the host cell. ADP-ribosylation factor-6 (ARF6) belongs to the ARF family of small GTP-binding proteins. ARF6 regulates membrane trafficking and actin cytoskeleton rearrangements at the plasma membrane. Here, we have observed that ARF6 is recruited to the parasitophorous vacuole of tachyzoites of T. gondii RH strain and it also plays an important role in the parasite cell invasion with activation of PI3-kinase and recruitment of PIP{sub 2} and PIP{sub 3} to the parasitophorous vacuole of invading parasites. Moreover, it was verified that maintenance of host cell actin cytoskeleton integrity is important to parasite invasion.
- OSTI ID:
- 21255843
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 378, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2008.11.108; PII: S0006-291X(08)02327-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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