Characterization of Pseudomonas chlororaphis myovirus 201{phi}2-1 via genomic sequencing, mass spectrometry, and electron microscopy
- Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900 (United States)
- Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602 (United States)
Pseudomonas chlororaphis phage 201{phi}2-1 is a relative of Pseudomonas aeruginosa myovirus {phi}KZ. Phage 201{phi}2-1 was examined by complete genomic sequencing (316,674 bp), by a comprehensive mass spectrometry survey of its virion proteins and by electron microscopy. Seventy-six proteins, of which at least 69 have homologues in {phi}KZ, were identified by mass spectrometry. Eight proteins, in addition to the major head, tail sheath and tail tube proteins, are abundant in the virion. Electron microscopy of 201{phi}2-1 revealed a multitude of long, fine fibers apparently decorating the tail sheath protein. Among the less abundant virion proteins are three homologues to RNA polymerase {beta} or {beta}' subunits. Comparison between the genomes of 201{phi}2-1 and {phi}KZ revealed substantial conservation of the genome plan, and a large region with an especially high rate of gene replacement. The {phi}KZ-like phages exhibited a two-fold higher rate of divergence than for T4-like phages or host genomes.
- OSTI ID:
- 21141009
- Journal Information:
- Virology, Vol. 376, Issue 2; Other Information: DOI: 10.1016/j.virol.2008.04.004; PII: S0042-6822(08)00214-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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