Small Angle X-ray Scattering in Structural Investigation of Selected Biological Systems
- Department of Macromolecular Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan (Poland)
Small angle X-ray scattering method (SAXS) is a technique complementary to the protein crystallography, allowing determination of the structural parameters such as the radius of gyration or the maximum size characterizing the macromolecules, and providing information on the conformational changes taking place in solution. The use of SAXS method enables a comparison of the protein crystal structure with the data collected in solution. Recent development of the measurement techniques (mainly those based on synchrotron radiation) and calculation methods has permitted introduction of advanced techniques also in the field of structural analysis of biomolecules (e.g. for determination of the shape of the protein molecule in solution). The paper presents a few selected methods of structural analysis of biological systems based on the SAXS data and illustrates their performance on the example of xylanase from Trichoderma longibrachiatum and a model phospholipid system.
- OSTI ID:
- 21039383
- Journal Information:
- AIP Conference Proceedings, Vol. 958, Issue 1; Conference: 4. International summer school on nuclear physics methods and accelerators in biology and medicine, Prague (Czech Republic), 8-19 Jul 2007; Other Information: DOI: 10.1063/1.2825775; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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