Pretreatment with soluble ST2 reduces warm hepatic ischemia/reperfusion injury
- Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 (China)
- Department of Surgery, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030 (China)
The interleukin-1 receptor-like protein ST2 exists in both membrane-bound (ST2L) and soluble form (sST2). ST2L has been found to play an important regulatory role in Th2-type immune response, but the function of soluble form of ST2 remains to be elucidated. In this study, we report the protective effect of soluble ST2 on warm hepatic ischemia/reperfusion injury. We constructed a eukaryotic expression plasmid, psST2-Fc, which expresses functional murine soluble ST2-human IgG1 Fc (sST2-Fc) fusion protein. The liver damage after ischemia/reperfusion was significantly attenuated by the expression of this plasmid in vivo. sST2-Fc remarkably inhibited the activation of Kupffer cells and the production of proinflammatory mediators TNF-{alpha} and IL-6. Furthermore, the levels of TLR4 mRNA and the nuclear translocation of NF-{kappa}B were also suppressed by pretreatment with sST2-Fc. These results thus identified soluble ST2 as a negative regulator in hepatic I/R injury, possibly via ST2-TLR4 pathway.
- OSTI ID:
- 20857940
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 351, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.10.166; PII: S0006-291X(06)02397-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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