Simultaneous suppression of lignin, tricin and wall‐bound phenolic biosynthesis via the expression of monolignol 4‐ O ‐methyltransferases in rice

Dwivedi, Nidhi; Yamamoto, Senri; Zhao, Yunjun; Hou, Guichuan; Bowling, Forrest; Tobimatsu, Yuki; Liu, Chang‐Jun

doi:10.1111/pbi.14186

Title: Simultaneous suppression of lignin, tricin and wall‐bound phenolic biosynthesis via the expression of monolignol 4‐ O ‐methyltransferases in rice

Journal Article · Thu Oct 05 00:00:00 EDT 2023 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.14186· OSTI ID:2007680

Dwivedi, Nidhi ^[1]; Yamamoto, Senri ^[2]; Zhao, Yunjun ^[3]; Hou, Guichuan ^[4]; Bowling, Forrest ^[3];

^[2];

^[1]

Biology Department Brookhaven Nation Laboratory Upton New York USA, Feedstocks Division Joint BioEnergy Institute Emeryville CA USA
Research Institute for Sustainable Humanosphere Kyoto University Gokasho, Uji Kyoto Japan
Biology Department Brookhaven Nation Laboratory Upton New York USA
Dewel Microscopy Facility Appalachian State University Boone North Carolina USA

Summary Grass lignocelluloses feature complex compositions and structures. In addition to the presence of conventional lignin units from monolignols, acylated monolignols and flavonoid tricin also incorporate into lignin polymer; moreover, hydroxycinnamates, particularly ferulate, cross‐link arabinoxylan chains with each other and/or with lignin polymers. These structural complexities make grass lignocellulosics difficult to optimize for effective agro‐industrial applications. In the present study, we assess the applications of two engineered monolignol 4‐ O ‐methyltransferases (MOMTs) in modifying rice lignocellulosic properties. Two MOMTs confer regiospecific para ‐methylation of monolignols but with different catalytic preferences. The expression of MOMTs in rice resulted in differential but drastic suppression of lignin deposition, showing more than 50% decrease in guaiacyl lignin and up to an 90% reduction in syringyl lignin in transgenic lines. Moreover, the levels of arabinoxylan‐bound ferulate were reduced by up to 50%, and the levels of tricin in lignin fraction were also substantially reduced. Concomitantly, up to 11 μmol/g of the methanol‐extractable 4‐ O ‐methylated ferulic acid and 5–7 μmol/g 4‐ O ‐methylated sinapic acid were accumulated in MOMT transgenic lines. Both MOMTs in vitro displayed discernible substrate promiscuity towards a range of phenolics in addition to the dominant substrate monolignols, which partially explains their broad effects on grass phenolic biosynthesis. The cell wall structural and compositional changes resulted in up to 30% increase in saccharification yield of the de‐starched rice straw biomass after diluted acid‐pretreatment. These results demonstrate an effective strategy to tailor complex grass cell walls to generate improved cellulosic feedstocks for the fermentable sugar‐based production of biofuel and bio‐chemicals.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Japan Society for the Promotion of Science (JSPS)

Grant/Contract Number:: DE‐AC02‐05CH11231; DE‐C0018420; DE‐SC0012704; SC0012704; AC02-05CH11231; JP20H03044; JP 22J13457

OSTI ID:: 2007680

Alternate ID(s):: OSTI ID: 2203256; OSTI ID: 2283251

Report Number(s):: BNL-224893-2023-JAAM

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 22 Journal Issue: 2; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Monolignol 4-O
methyltransferase
lignin
wall-bound phenolics
tricin
4-O-methylated
ferulic acid
saccharification
Oryza sativa
4-O-methylated sinapic acid
p-coumaric acid

Title: Simultaneous suppression of lignin, tricin and wall‐bound phenolic biosynthesis via the expression of monolignol 4‐ O ‐methyltransferases in rice

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